Article Dans Une Revue International Journal of Antimicrobial Agents Année : 2010

Purification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by n.v. Er 317/402 strain Narine

Résumé

In the last two decades, antimicrobial peptides (AMPs) have been gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. n.v. Er 317/402 strain Narine produces a small AMP with a molecular weight of 1.1kDa, designated acidocin LCHV. In this study, the AMP was extremely heat stable (90min at 130°C), was active over a wide pH range and was found to be sensitive to proteolytic enzymes (trypsin, pepsin and proteinase K). Acidocin LCHV has a broad spectrum of activity both against Gram-positive and Gram-negative pathogens, including several that are classified as Especially Dangerous Infections by the World Health Organization as well as meticillin-resistant (MRSA) and . Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) was used to determine the molecular mass and sequence of the purified peptide. Complete killing with immediate impact on cells was observed within a very short period of time (10min).

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hal-00556387 , version 1 (16-01-2011)

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Hermine Mkrtchyan, Simon Gibbons, Sibylle Heidelberger, Mire Zloh, Hamidreza Khalatbari Limaki. Purification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by n.v. Er 317/402 strain Narine. International Journal of Antimicrobial Agents, 2010, 35 (3), pp.255. ⟨10.1016/j.ijantimicag.2009.11.017⟩. ⟨hal-00556387⟩

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