Age-related Purkinje cell death is steroid dependent: RORα haplo-insufficiency impairs plasma and cerebellar steroids and Purkinje cell survival. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Age (Dordrecht, Netherlands) Année : 2011

Age-related Purkinje cell death is steroid dependent: RORα haplo-insufficiency impairs plasma and cerebellar steroids and Purkinje cell survival.

Yvette Akwa
  • Fonction : Auteur
Joëlle Bakouche
  • Fonction : Auteur
  • PersonId : 889059
Philippe Liere
  • Fonction : Auteur
Bernard Eychenne
  • Fonction : Auteur
Antoine Pianos
  • Fonction : Auteur
Paul Luiten
  • Fonction : Auteur
Ton Groothuis
  • Fonction : Auteur
Etienne-Emile Baulieu
  • Fonction : Auteur
Jean Mariani
  • Fonction : Auteur
  • PersonId : 858634
Rachel M Sherrard
  • Fonction : Auteur
  • PersonId : 879043

Résumé

A major problem of ageing is progressive impairment of neuronal function and ultimately cell death. Since sex steroids are neuroprotective, their decrease with age may underlie age-related neuronal degeneration. To test this, we examined Purkinje cell numbers, plasma sex steroids and cerebellar neurosteroid concentrations during normal ageing (wild-type mice, WT), in our model of precocious ageing (Rora ( +/sg ), heterozygous staggerer mice in which expression of the neuroprotective factor RORα is disrupted) and after long-term hormone insufficiency (WT post-gonadectomy). During normal ageing (WT), circulating sex steroids declined prior to or in parallel with Purkinje cell loss, which began at 18 months of age. Although Purkinje cell death was advanced in WT long-term steroid deficiency, this premature neuronal loss did not begin until 9 months, indicating that vulnerability to sex steroid deficiency is a phenomenon of ageing Purkinje neurons. In precocious ageing (Rora ( +/sg )), circulating sex steroids decreased prematurely, in conjunction with marked Purkinje cell death from 9 months. Although Rora ( +/sg ) Purkinje cells are vulnerable through their RORα haplo-insufficiency, it is only as they age (after 9 months) that sex steroid failure becomes critical. Finally, cerebellar neurosteroids did not decrease with age in either genotype or gender; but were profoundly reduced by 3 months in male Rora ( +/sg ) cerebella, which may contribute to the fragility of their Purkinje neurons. These data suggest that ageing Purkinje cells are maintained by circulating sex steroids, rather than local neurosteroids, and that in Rora ( +/sg ) their age-related death is advanced by premature sex steroid loss induced by RORα haplo-insufficiency.

Domaines

Neurosciences

Dates et versions

hal-00555453 , version 1 (13-01-2011)

Identifiants

Citer

Sonja Janmaat, Yvette Akwa, Mohamed Doulazmi, Joëlle Bakouche, Vanessa Gautheron, et al.. Age-related Purkinje cell death is steroid dependent: RORα haplo-insufficiency impairs plasma and cerebellar steroids and Purkinje cell survival.. Age (Dordrecht, Netherlands), 2011, 33 (4), pp.565-578. ⟨10.1007/s11357-010-9203-3⟩. ⟨hal-00555453⟩
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