A preliminary study of heterotrophic picoflagellates using oligonucleotidic probes in Lake Pavin - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Hydrobiologia Année : 2005

A preliminary study of heterotrophic picoflagellates using oligonucleotidic probes in Lake Pavin

Résumé

Free-living heterotrophic flagellates have been widely studied as key intermediates in aquatic food webs. However, very few attempts have been made (all in marine systems) to study the smallest cells belonging to these communities. In this study, we report on the quantitative importance and the vertical distribution of heterotrophic picoflagellates during spring in the oligomesotrophic Lake Pavin, using the FISH approach. The rRNA-based probes used, initially designed for two novel lineages of heterotrophic picoflagellates (i.e., the Stramenopiles NS3 and NS4) in marine systems, revealed high abundances (in the order of 104 cells ml-1 and up to about 6 x 104 cells ml-1) of the targeted picoflagellates in Lake Pavin, i.e. one order of magnitude higher than in seawater samples. 'NS3' cells were more often detected than 'NS4' cells, the average contributions of the two populations to the total abundance of heterotrophic flagellates being at 25 and 9%, respectively. However, the probes used were apparently non-specific to the targeted cells, implying that the occurrence of the two novel lineages NS3 and NS4 of Stramenopiles in freshwaters needs to be confirmed. Although, this study clearly confirms the occurrence, in high abundance, of small heterotrophic eukaryotes in Lake Pavin. In a near future, we believe that the availability of microbial clone libraries will allow us to improve our knowledge of the composition and the functional role of small heterotrophic eukaryotes in freshwaters.
Fichier non déposé

Dates et versions

hal-00527490 , version 1 (19-10-2010)

Identifiants

  • HAL Id : hal-00527490 , version 1

Citer

Emilie Lefèvre, Jean-François Carrias, Corinne Bardot, Télesphore Sime-Ngando, Christian Amblard. A preliminary study of heterotrophic picoflagellates using oligonucleotidic probes in Lake Pavin. Hydrobiologia, 2005, 551, pp.61-67. ⟨hal-00527490⟩
87 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More