A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Diagnostic Microbiology and Infectious Disease Année : 2009

A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis.

Andrea Ciammaruconi
  • Fonction : Auteur
Saverio Grassi
  • Fonction : Auteur
Giovanni Faggioni
  • Fonction : Auteur
Riccardo de Santis
  • Fonction : Auteur
Valentina Pittiglio
  • Fonction : Auteur
Raffaele d'Amelio
  • Fonction : Auteur
Florigio Lista
  • Fonction : Auteur

Résumé

Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing technique permits to differentiate Y. pestis isolates and turned out to be robust, reproducible, and cheap.

Dates et versions

hal-00527486 , version 1 (19-10-2010)

Identifiants

Citer

Andrea Ciammaruconi, Saverio Grassi, Giovanni Faggioni, Riccardo de Santis, Valentina Pittiglio, et al.. A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis.. Diagnostic Microbiology and Infectious Disease, 2009, 65 (1), pp.7-13. ⟨10.1016/j.diagmicrobio.2009.04.015⟩. ⟨hal-00527486⟩
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