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Article Dans Une Revue Nature Methods Année : 2010

Imaging plant growth in 4D : robust tissue reconstruction and lineaging at cell resolution.

Résumé

Quantitative information on growing organs is required to better understand morphogenesis in both plants and animals. However, detailed analyses of growth patterns at cellular resolution have remained elusive. We developed an approach, multiangle image acquisition, three-dimensional reconstruction and cell segmentation-automated lineage tracking (MARS-ALT), in which we imaged whole organs from multiple angles, computationally merged and segmented these images to provide accurate cell identification in three dimensions and automatically tracked cell lineages through multiple rounds of cell division during development. Using these methods, we quantitatively analyzed Arabidopsis thaliana flower development at cell resolution, which revealed differential growth patterns of key regions during early stages of floral morphogenesis. Lastly, using rice roots, we demonstrated that this approach is both generic and scalable.
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Dates et versions

hal-00521491 , version 1 (27-09-2010)

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R. Fernandez, Pradeep Das, V. Mirabet, E. Moscardi, Jan Traas, et al.. Imaging plant growth in 4D : robust tissue reconstruction and lineaging at cell resolution.. Nature Methods, 2010, 7, pp.547-553. ⟨10.1038/NMETH.1472⟩. ⟨hal-00521491⟩
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