Assessment of the biochemical composition of oilseed rape (Brassica napus L.) C-13-labelled residues by global methods, FTIR and C-13 NMR CP/MAS - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant and Soil Année : 2001

Assessment of the biochemical composition of oilseed rape (Brassica napus L.) C-13-labelled residues by global methods, FTIR and C-13 NMR CP/MAS

Résumé

The biochemical composition of stems, pod walls and roots of oilseed rape (Brassica napus L.) plants, grown in a growth chamber with two levels of N fertiliser, was assessed by two global methods, i.e., serial extraction with the Van Soest's technique and temperature-programmed pyroanalysis (TP-Py). Statistical analysis of the effect of various parameters on the proportion of soluble components, hemicellulose, cellulose and lignin-like components in oilseed rape organs showed that the composition of plant materials depended on the N nutrition conditions during plant growth. Contents of soluble and hemicellulose fractions were affected by the technique used. Elsewhere, both global techniques resulted in similar proportions of skeletal cellulose (respectively 41 and 36% in low and high N stems, 37 and 30% in low and high N pod walls, 32 and 29% in low and high N roots) and of lignin-like components which ranged from about 7% in high N stems and pod walls to 16% in low N roots. Spectroscopy by FTIR showed a significant band at 1650 cm(-1) (amide I in proteins) in the root material (organ with the lowest C/N ratio) and the absence of lignin-specific bands. Carbon distribution by C-13 NMR CP/MAS of labelled plants indicated that 60-64% was (cellulose + hemicellulose)-C, close to the values obtained by global methods. The proportion of aromatic-C (110-160 ppm) and phenolic ether was higher in roots than in stems and pod walls. Organs from oilseed rape plants with higher N contents exhibited a larger proportion of C in the 171 ppm chemical shift attributed to the peptide bond. The concomitance of a high level of aromatic and proteinaceous components in roots would reveal the presence of tannin-protein complexes in addition with true lignin.

Dates et versions

hal-00511247 , version 1 (24-08-2010)

Identifiants

Citer

I. Trinsoutrot, Lucile Jocteur-Monrozier, J. Cellier, Hughes Waton, Serge Alamercery, et al.. Assessment of the biochemical composition of oilseed rape (Brassica napus L.) C-13-labelled residues by global methods, FTIR and C-13 NMR CP/MAS. Plant and Soil, 2001, 234 (1), pp.61-72. ⟨10.1023/A:1010549224003⟩. ⟨hal-00511247⟩
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