Multi-residue analysis of steroids at sub-ng/L levels in surface and ground-waters using liquid chromatography coupled to tandem mass spectrometry.
Résumé
Most analytical methodologies currently available for the determination of steroids in water only identify a few representative compounds (mainly estrogens). In this context, a multi-residue methodology based on liquid chromatography-tandem mass spectrometry was developed for the determination of 26 steroids including natural and synthetic estrogens, progestagens and androgens. The method described involves limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using a C18 cartridge. The analytical procedure allows the determination of the target analytes in the lower ng/L range, with recoveries above 80%. The methodology was successfully applied to the analysis of steroids in several surface and ground-waters. In all the waters, estrogens, androgens and/or progestagens were determined.
Mots clés
- Water analysis
- Androgen
- Estrogen
- Elution
- Electrospray
- Trace analysis
- Solid phase extraction
- Gradient
- Quantitative analysis
- Ground water
- Steroid
- Multicomponent analysis
- Surface water
- Progestagen
- Water pollution
- Coupled method
- HPLC chromatography
- Chemical analysis
- Water quality
- Chemical enrichment
- Sample preparation
- Tandem mass spectrometry
- LC-MS/MS
- Surface and ground-waters analysis
- Steroids