Hypoxia induces caspase-9 and caspase-3 activation without neuronal death in gerbil brains. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue European Journal of Neuroscience Année : 2004

Hypoxia induces caspase-9 and caspase-3 activation without neuronal death in gerbil brains.

Anne Prigent-Tessier
  • Fonction : Auteur
Michaël van Hoecke
  • Fonction : Auteur
Nathalie Bertrand
  • Fonction : Auteur
Olivier Sordet
  • Fonction : Auteur
Raymond A Swanson
  • Fonction : Auteur
Christine Marie
  • Fonction : Auteur
Alain Beley
  • Fonction : Auteur

Résumé

To investigate the in vivo apoptotic machinery in oxygen deprived brain, we examined the expression of caspase-9 and caspase-3 in the hippocampus of Mongolian gerbils subjected to either transient hypoxia (4% O2 for 6 min) or forebrain ischemia (10 min bilateral carotid artery occlusion) followed by 8 h to 7 days of reoxygenation or blood recirculation. Apoptotic death was characterized by isolating hippocampal genomic DNA and analysing DNA fragmentation as well as histological studies including TUNEL assay and toluidine blue staining of brain sections. The results showed that both hypoxic and ischemic gerbil brains exhibited an increase in caspase-9 and caspase-3 gene expression. However, no cell damage was detectable following hypoxia, while marked DNA fragmentation and extensive cell death was observed following ischemia. Moreover, although hypoxia did not lead to cell death, both hypoxia and ischemia were associated with cleavage of procaspase-9 and procaspase-3 and increases in their activities as well as cleavage of poly(ADP-ribose) polymerase-1 (PARP-1), a major caspase-3 substrate. These results indicate that, in vivo, even late apoptotic events such as caspase activation and PARP-1 cleavage in hypoxic brains do not necessarily induce an irreversible commitment to apoptotic neuronal death.

Dates et versions

hal-00499522 , version 1 (09-07-2010)

Identifiants

Citer

Philippe Garnier, Anne Prigent-Tessier, Michaël van Hoecke, Nathalie Bertrand, Céline Demougeot, et al.. Hypoxia induces caspase-9 and caspase-3 activation without neuronal death in gerbil brains.. European Journal of Neuroscience, 2004, 20 (4), pp.937-946. ⟨10.1111/j.1460-9568.2004.03551.x⟩. ⟨hal-00499522⟩
93 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More