Role of TI-VAMP and CD82 in EGFR cell-surface dynamics and signaling. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2010

Role of TI-VAMP and CD82 in EGFR cell-surface dynamics and signaling.

Lydia Danglot
Franck Perez
Thierry Galli

Résumé

The v-SNARE TI-VAMP (VAMP7) mediates exocytosis during neuritogenesis, phagocytosis and lysosomal secretion. It localizes to endosomes and lysosomes but also to the trans-Golgi network. Here we show that depletion of TI-VAMP enhances the endocytosis of activated EGF receptor (EGFR) without affecting constitutive endocytosis of EGFR, or transferrin uptake. This increased EGFR internalization is mainly clathrin dependent. Searching for defects in EGFR regulators, we found that TI-VAMP depletion reduces the cell surface amount of CD82, a tetraspanin known to control EGFR localization in microdomains. We further show that TI-VAMP is required for secretion from the Golgi apparatus to the cell surface, and that TI-VAMP-positive vesicles transport CD82. Quantum dots video-microscopy indicates that depletion of TI-VAMP, or its cargo CD82, restrains EGFR diffusion and the area explored by EGFR at the cell surface. Both depletions also impair MAPK signaling and enhance endocytosis of activated EGFR by increased recruitment of AP-2. These results highlight the role of TI-VAMP in the secretory pathway of a tetraspanin, and support a model in which CD82 allows EGFR entry in microdomains that control its clathrin-dependent endocytosis and signaling.
Fichier non déposé

Dates et versions

hal-00486387 , version 1 (25-05-2010)

Identifiants

Citer

Lydia Danglot, Mathilde Chaineau, Maxime Dahan, Marie-Claude Gendron, Nicole Boggetto, et al.. Role of TI-VAMP and CD82 in EGFR cell-surface dynamics and signaling.. Journal of Cell Science, 2010, 123 (Pt 5), pp.723-35. ⟨10.1242/jcs.062497⟩. ⟨hal-00486387⟩
129 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More