Article Dans Une Revue Biochemical Journal Année : 2008

Negative regulation of cell cycle progression by RINGO/Speedy E

Ana Dinarina
  • Fonction : Auteur
E. Josué Ruiz
  • Fonction : Auteur
Ana O'Loghlen
  • Fonction : Auteur
Silvana Mouron
  • Fonction : Auteur
Laurent Perez
  • Fonction : Auteur

Résumé

Cell cycle transitions are controlled by cyclin-dependent kinases (CDKs), whose activation is usually associated with the binding of cyclins. RINGO/Speedy proteins can also bind to and activate CDKs, although they do not have amino acid sequence homology to cyclins. The RINGO/Speedy family members studied so far positively regulate cell cycle progression. Here we report the biochemical and functional characterization of RINGO/Speedy E. We show that RINGO/Speedy E is a functionally distant member of this protein family that negatively affects cell cycle progression. RINGO/Speedy E overexpression inhibits the meiotic progression in Xenopus oocytes as well as the proliferation of mammalian cells. RINGO/Speedy E can bind to endogenous CDK1 and CDK2 in both cellular systems. However, the RINGO/Speedy E-activated CDKs have different substrate specificity than the CDKs activated by other RINGO/Speedy proteins, which may account for their different effects on the cell cycle. Our results indicate that although all RINGO/Speedy family members can activate CDKs, they may differently regulate cell cycle progression.

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hal-00478900 , version 1 (30-04-2010)

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Ana Dinarina, E. Josué Ruiz, Ana O'Loghlen, Silvana Mouron, Laurent Perez, et al.. Negative regulation of cell cycle progression by RINGO/Speedy E. Biochemical Journal, 2008, 410 (3), pp.535-542. ⟨10.1042/BJ20071453⟩. ⟨hal-00478900⟩

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