Detection of 13, 19-didesmethyl C spirolide by fluorescence polarization using Torpedo electrocyte membranes. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Analytical Biochemistry Année : 2010

Detection of 13, 19-didesmethyl C spirolide by fluorescence polarization using Torpedo electrocyte membranes.

Résumé

Fluorescence polarization (FP) is a powerful tool for studying molecular interactions by monitoring changes in the apparent size of fluorescent molecules. In this paper, a previously described fluorescence polarization assay was used to detect 13,19-didesmethyl C spirolide. The assay is based on the competition of cyclic imine marine biotoxins with alpha-bungarotoxin for binding to nicotinic acetylcholine receptor-enriched membranes of Torpedo marmorata. The 13,19-didesmethyl C spirolide was detected in buffer and mussel matrix, The sensitivity of the assay for the 13,19-didesmethyl C spirolide and the 13-desmethyl C spirolide was similar. After an acetone/chloroform extraction of spiked mussel meat, the average recovery rate of 13,19-didesmethyl C spirolide was 77.7% +/- 1.9%. The quantification range for this toxin in mussel was 40-200 mug/kg of shellfish meat. This assay can be used to detect the spirolides 13,19-didesmethyl C spirolide and 13-desmethyl C spirolide, in shellfish as a screening assay.

Dates et versions

hal-00474310 , version 1 (19-04-2010)

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Citer

Eva S Fonfría, Natalia Vilariño, Jordi Molgó, Rómulo Aráoz, Paz Otero, et al.. Detection of 13, 19-didesmethyl C spirolide by fluorescence polarization using Torpedo electrocyte membranes.. Analytical Biochemistry, 2010, 403 (1-2), pp.102-7. ⟨10.1016/j.ab.2010.04.006⟩. ⟨hal-00474310⟩

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