The peripheral nerve and the neuromuscular junction are affected in the tenascin-C-deficient mouse. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cellular and Molecular Biology Année : 1998

The peripheral nerve and the neuromuscular junction are affected in the tenascin-C-deficient mouse.

Résumé

A thorough examination of the structure and plasticity of the neuromuscular system was performed in tenascin-C mutant mice deficient in tenascin-C. The study of the peripheral nerve revealed a number of abnormal features. In the motor nerve, numerous unmyelinated and myelinated fibers with degraded myelin were present. Schwann cell processes often enclosed degenerative terminals. Transgene (beta-galactosidase) expression analyzed at the ultrastructural level was found to be unequally distributed in the mutant's neuromuscular tissues. At the NMJ, preterminal disorganization was prevalent. Some axon terminals exhibited abnormal overgrowth. A surprising lack of beta-galactosidase expression at some cellular sites known to possess tenascin-C in wild type mice correlated best with marked changes in the cytoarchitecture of the peripheral nerve and NMJ. In some other -but not all- cellular sites which normally express the molecule, immunofluorescence analysis suggested the presence of significant but low levels of tenascin-C-like immunoreactivity together with beta-galactosidase expression. Messenger RNA detection by RT-PCR confirmed the presence of low amounts of tenascin-C mRNA in skeletal muscle suggesting that the mice deficient in tenascin-C are not complete knock-outs of this gene, but low-expression mutants. Following in vivo injections of botulinum type-A toxin, we observed a greatly reduced sprouting response of the motor nerves in tenascin-C mutant mice. We also observed that N-CAM and beta-catenin were overexpressed in the mutant. Our results suggest that tenascin-C is involved both in stabilization and in plasticity of the NMJ.
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Dates et versions

hal-00471168 , version 1 (07-04-2010)

Identifiants

  • HAL Id : hal-00471168 , version 1
  • PUBMED : 9593587

Citer

C. Cifuentes-Diaz, E. Velasco, Frédéric A. Meunier, D. Goudou, L. Belkadi, et al.. The peripheral nerve and the neuromuscular junction are affected in the tenascin-C-deficient mouse.. Cellular and Molecular Biology, 1998, 44 (2), pp.357-79. ⟨hal-00471168⟩

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