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Journal Articles Biophysical Journal Year : 2009

Quantitative Imaging of Molecular Order in Lipid Membranes Using Two-Photon Fluorescence Polarimetry

Alecja Gasecka
  • Function : Author
Tsai Jung Han
  • Function : Author
Cyril Favard
Bong Rae Cho
  • Function : Author
Sophie Brasselet
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Abstract

We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains.

Dates and versions

hal-00455205 , version 1 (09-02-2010)

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Alecja Gasecka, Tsai Jung Han, Cyril Favard, Bong Rae Cho, Sophie Brasselet. Quantitative Imaging of Molecular Order in Lipid Membranes Using Two-Photon Fluorescence Polarimetry. Biophysical Journal, 2009, 97 (10), pp.2854. ⟨10.1016/j.bpj.2009.08.052⟩. ⟨hal-00455205⟩
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