Factorial design optimization and in vivo feasibility of poly(ε-caprolactone)-micro- and nanofiber-based small diameter vascular grafts. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biomedical Materials Research Part A Année : 2009

Factorial design optimization and in vivo feasibility of poly(ε-caprolactone)-micro- and nanofiber-based small diameter vascular grafts.

Résumé

Because of the severe increase of mortality by cardiovascular diseases, there has been rising interest among the tissue-engineering community for small-sized blood vessel substitutes. Here we present small diameter vascular grafts made of slow degradable poly(epsilon-caprolactone) nanofibers obtained by electrospinning. The process was optimized by a factorial design approach that led to reproducible grafts with inner diameters of 2 and 4 mm, respectively. Fiber sizes, graft morphology, and the resulting tensile stress and tensile strain values were studied as a function of various parameters in order to obtain optimal vascular grafts for implantation after gamma-sterilization. The influence of polymer concentration, solvent, needle-collector distance, applied voltage, flow rate, and spinning time has been studied. Consequently, an optimized vascular graft was implanted as an abdominal aortic substitute in nine rats for a feasibility study. Results are given following up a 12-week implantation period showing good patency, endothelization, and cell ingrowth.
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Dates et versions

hal-00416778 , version 1 (12-01-2024)

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Benjamin Nottelet, Erman Pektok, Delia Mandracchia, Jean-Christophe Tille, Beat H. Walpoth, et al.. Factorial design optimization and in vivo feasibility of poly(ε-caprolactone)-micro- and nanofiber-based small diameter vascular grafts.. Journal of Biomedical Materials Research Part A, 2009, 89 (4), pp.865-75. ⟨10.1002/jbm.a.32023⟩. ⟨hal-00416778⟩
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