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Article Dans Une Revue Nature Methods Année : 2009

SUnSET, a nonradioactive method to monitor protein synthesis.

Résumé

We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.

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Immunologie
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Dates et versions

hal-00408410 , version 1 (30-07-2009)

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Enrico K Schmidt, Giovanna Clavarino, Maurizio Ceppi, Philippe Pierre. SUnSET, a nonradioactive method to monitor protein synthesis.. Nature Methods, 2009, 6 (4), pp.275-7. ⟨10.1038/nmeth.1314⟩. ⟨hal-00408410⟩

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