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Article Dans Une Revue Review of Scientific Instruments Année : 2009

A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell

Patrick Ferrand
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M. Pianta
  • Fonction : Auteur
A. Kress
  • Fonction : Auteur
A. Aillaud
  • Fonction : Auteur
H. Rigneault

Résumé

A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes, are reported.
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Dates et versions

hal-00408227 , version 1 (29-07-2009)

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Patrick Ferrand, M. Pianta, A. Kress, A. Aillaud, H. Rigneault, et al.. A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell. Review of Scientific Instruments, 2009, 80 (8), pp.083702. ⟨10.1063/1.3205447⟩. ⟨hal-00408227⟩
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