A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell - Archive ouverte HAL Access content directly
Journal Articles Review of Scientific Instruments Year : 2009

A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell

Patrick Ferrand
Connectez-vous pour contacter l'auteur
M. Pianta
  • Function : Author
A. Kress
  • Function : Author
A. Aillaud
  • Function : Author
H. Rigneault

Abstract

A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes, are reported.
Fichier principal
Vignette du fichier
dualFCS.pdf (426.34 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-00408227 , version 1 (29-07-2009)

Identifiers

Cite

Patrick Ferrand, M. Pianta, A. Kress, A. Aillaud, H. Rigneault, et al.. A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell. Review of Scientific Instruments, 2009, 80 (8), pp.083702. ⟨10.1063/1.3205447⟩. ⟨hal-00408227⟩
74 View
195 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More