H2O2 activates the nuclear localization of Msn2 and Maf1 through thioredoxins in yeast. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Eukaryotic Cell Année : 2009

H2O2 activates the nuclear localization of Msn2 and Maf1 through thioredoxins in yeast.

Résumé

The cellular response to hydrogen peroxide (H2O2) is characterized by a repression of growth-related processes and an enhanced expression of genes important for cell defense. In budding yeast, this response requires the activation of a set of transcriptional effectors. Some of them, such as the transcriptional activator Yap1, are specific to oxidative stress and others, such as the transcriptional activators Msn2/4 and the negative regulator Maf1, are activated by a wide spectrum of stress conditions. How these general effectors are activated in response to oxidative stress remains an open question. In this study, we demonstrate that the two cytoplasmic thioredoxins, Trx1 and Trx2, are essential to trigger the nuclear accumulation of Msn2/4 and Maf1, specifically under H2O2 treatment. Contrarily to many stress conditions previously described in yeast, the H2O2-induced nuclear accumulation of Msn2 and Maf1 does not correlate with the down-regulation of PKA kinase activity. Nevertheless, we show that the PP2A phosphatase activity is essential for driving Maf1 dephosphorylation and its subsequent nuclear accumulation in response to H2O2 treatment. Interestingly, under this condition, the lack of PP2A activity has no impact on the subcellular localization of Msn2, demonstrating that the H2O2 signaling pathways share a common route through the thioredoxin system and then diverge to activate Msn2 and Maf1, the final integrators of these pathways.

Dates et versions

hal-00404476 , version 1 (16-07-2009)

Identifiants

Citer

Stéphanie Boisnard, Gilles Lagniel, Cecilia Garmendia-Torres, Mikael Molin, Emmanuelle Boy-Marcotte, et al.. H2O2 activates the nuclear localization of Msn2 and Maf1 through thioredoxins in yeast.. Eukaryotic Cell, 2009, epub ahead of print. ⟨10.1128/EC.00106-09⟩. ⟨hal-00404476⟩
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