Brilliant molecular nanocrystals emerging from sol-gel thin films: towards a new generation of fluorescent biochips. - Archive ouverte HAL Access content directly
Journal Articles Nanotechnology Year : 2009

Brilliant molecular nanocrystals emerging from sol-gel thin films: towards a new generation of fluorescent biochips.

Emilie Dubuisson
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Virginie Monnier
B. Boury
Yves Usson
Alain Ibanez

Abstract

To develop highly sensitive biosensors, we made directly available to biological aqueous solutions organic nanocrystals previously grown in the pores of sol-gel films. Through the controlled dissolution of the sol-gel surface, we obtained emerging nanocrystals that remained strongly anchored to the sol-gel coating for good mechanical stability of the final sensing device. We demonstrated that in the presence of a solution of DNA functionalized with a molecular probe, the nanocrystal fluorescence is strongly quenched by Förster resonance energy transfer thus opening the way towards very sensitive fluorescent biosensors through biomolecules grafted onto fluorescent nanocrystals. Finally, this controlled dissolution, involving weak concentrated NaOH solution, is a generic process that can be used for the thinning of any kind of sol-gel layer.

Dates and versions

hal-00404061 , version 1 (15-07-2009)

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Emilie Dubuisson, Virginie Monnier, Nathalie Sanz-Menez, B. Boury, Yves Usson, et al.. Brilliant molecular nanocrystals emerging from sol-gel thin films: towards a new generation of fluorescent biochips.. Nanotechnology, 2009, 20 (31), pp.315301. ⟨10.1088/0957-4484/20/31/315301⟩. ⟨hal-00404061⟩
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