Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens. - Archive ouverte HAL
Article Dans Une Revue Methods in Molecular Biology Année : 2009

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens.

Résumé

Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA sequences composed of a succession of repeats (23- to 47-bp long) separated by unique sequences called spacers. Polymorphism can be observed in different strains of a species and may be used for genotyping. We describe protocols and bioinformatics tools that allow the identification of CRISPRs from sequenced genomes, their comparison, and their component determination (the direct repeats and the spacers). A schematic representation of the spacer organization can be produced, allowing an easy comparison between strains.
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Dates et versions

hal-00397745 , version 1 (23-06-2009)

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Ibtissem Grissa, Gilles Vergnaud, Christine Pourcel. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens.. Methods in Molecular Biology, 2009, 551, pp.105-16. ⟨10.1007/978-1-60327-999-4_9⟩. ⟨hal-00397745⟩
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