<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-00395454</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-17T02:51:17+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Effect of dietary cholesterol on low density lipoprotein-receptor, 3-hydroxy-3-methylglutaryl-CoA reductase, and low density lipoprotein receptor-related protein mRNA expression in healthy humans.</title>
            <author role="aut">
              <persName>
                <forename type="first">P.</forename>
                <surname>Boucher</surname>
              </persName>
              <idno type="halauthorid">218791-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">M.</forename>
                <surname>de Lorgeril</surname>
              </persName>
              <idno type="halauthorid">399953-0</idno>
              <affiliation ref="#struct-707"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">P.</forename>
                <surname>Salen</surname>
              </persName>
              <idno type="halauthorid">399954-0</idno>
              <affiliation ref="#struct-707"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">P.</forename>
                <surname>Crozier</surname>
              </persName>
              <idno type="halauthorid">400012-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">J.</forename>
                <surname>Delaye</surname>
              </persName>
              <idno type="halauthorid">290108-0</idno>
              <affiliation ref="#struct-40056"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">J. J.</forename>
                <surname>Vallon</surname>
              </persName>
              <idno type="halauthorid">400013-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">A.</forename>
                <surname>Geyssant</surname>
              </persName>
              <idno type="halauthorid">400005-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">R.</forename>
                <surname>Dante</surname>
              </persName>
              <idno type="halauthorid">400014-0</idno>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Patricia</forename>
                <surname>Salen</surname>
              </persName>
              <email type="md5">5d809932810bd27b18002a40e073067e</email>
              <email type="domain">ujf-grenoble.fr.invalid</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2009-06-15 16:08:35</date>
              <date type="whenModified">2025-09-27 20:00:18</date>
              <date type="whenReleased">2009-06-15 16:08:35</date>
              <date type="whenProduced">1998-12</date>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="140373">
                <persName>
                  <forename>Patricia</forename>
                  <surname>Salen</surname>
                </persName>
                <email type="md5">5d809932810bd27b18002a40e073067e</email>
                <email type="domain">ujf-grenoble.fr.invalid</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-00395454</idno>
            <idno type="halUri">https://hal.science/hal-00395454</idno>
            <idno type="halBibtex">boucher:hal-00395454</idno>
            <idno type="halRefHtml">&lt;i&gt;Lipids&lt;/i&gt;, 1998, 33 (12), pp.1177-86</idno>
            <idno type="halRef">Lipids, 1998, 33 (12), pp.1177-86</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CEA">CEA - Commissariat à l'énergie atomique</idno>
            <idno type="stamp" n="UGA">HAL Grenoble Alpes</idno>
            <idno type="stamp" n="IMAG">IMAG</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-GRENOBLE1">Université Joseph Fourier - Grenoble I</idno>
            <idno type="stamp" n="INPG">Institut polytechnique de Grenoble</idno>
            <idno type="stamp" n="TIMC-IMAG">TIMC</idno>
            <idno type="stamp" n="TIMC-IMAG-PRETA" corresp="TIMC-IMAG">PRETA : Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée</idno>
            <idno type="stamp" n="DEN" corresp="CEA">Direction des énergies</idno>
            <idno type="stamp" n="DEN-MARCO" corresp="CEA">DES Marcoule</idno>
            <idno type="stamp" n="UNIV-LYON">Université de Lyon</idno>
            <idno type="stamp" n="DE2D" corresp="DEN">Département de recherche sur les Procédés et Matériaux pour les Environnements complexes (Nouveau)</idno>
            <idno type="stamp" n="TEST3-HALCNRS">TEST3-HALCNRS</idno>
            <idno type="stamp" n="ISEC" corresp="DEN">l’Institut des sciences et technologies pour une économie circulaire des énergies bas carbone (ISEC)</idno>
            <idno type="stamp" n="TEST-UGA">TEST-UGA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Effect of dietary cholesterol on low density lipoprotein-receptor, 3-hydroxy-3-methylglutaryl-CoA reductase, and low density lipoprotein receptor-related protein mRNA expression in healthy humans.</title>
                <author role="aut">
                  <persName>
                    <forename type="first">P.</forename>
                    <surname>Boucher</surname>
                  </persName>
                  <idno type="halauthorid">218791-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">M.</forename>
                    <surname>de Lorgeril</surname>
                  </persName>
                  <idno type="halauthorid">399953-0</idno>
                  <affiliation ref="#struct-707"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">P.</forename>
                    <surname>Salen</surname>
                  </persName>
                  <idno type="halauthorid">399954-0</idno>
                  <affiliation ref="#struct-707"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">P.</forename>
                    <surname>Crozier</surname>
                  </persName>
                  <idno type="halauthorid">400012-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">J.</forename>
                    <surname>Delaye</surname>
                  </persName>
                  <idno type="halauthorid">290108-0</idno>
                  <affiliation ref="#struct-40056"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">J. J.</forename>
                    <surname>Vallon</surname>
                  </persName>
                  <idno type="halauthorid">400013-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">A.</forename>
                    <surname>Geyssant</surname>
                  </persName>
                  <idno type="halauthorid">400005-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">R.</forename>
                    <surname>Dante</surname>
                  </persName>
                  <idno type="halauthorid">400014-0</idno>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">16768</idno>
                <idno type="issn">0024-4201</idno>
                <idno type="eissn">1558-9307</idno>
                <title level="j">Lipids</title>
                <imprint>
                  <publisher>Springer Verlag</publisher>
                  <biblScope unit="volume">33</biblScope>
                  <biblScope unit="issue">12</biblScope>
                  <biblScope unit="pp">1177-86</biblScope>
                  <date type="datePub">1998-12</date>
                </imprint>
              </monogr>
              <idno type="pubmed">9930403</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="mesh">Adult</classCode>
              <classCode scheme="mesh">Base Sequence</classCode>
              <classCode scheme="mesh">RNA, Messenger</classCode>
              <classCode scheme="mesh">Receptors, Immunologic</classCode>
              <classCode scheme="mesh">Receptors, LDL</classCode>
              <classCode scheme="mesh">Reference Values</classCode>
              <classCode scheme="mesh">Cholesterol, Dietary</classCode>
              <classCode scheme="mesh">DNA Primers</classCode>
              <classCode scheme="mesh">Fatty Acids</classCode>
              <classCode scheme="mesh">Humans</classCode>
              <classCode scheme="mesh">Hydroxymethylglutaryl CoA Reductases</classCode>
              <classCode scheme="mesh">LDL-Receptor Related Protein 1</classCode>
              <classCode scheme="mesh">Male</classCode>
              <classCode scheme="mesh">Middle Aged</classCode>
              <classCode scheme="halDomain" n="sdv.aen">Life Sciences [q-bio]/Food and Nutrition</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>We investigated the possibility that dietary cholesterol downregulates the expression of low density lipoprotein (LDL) receptor and 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase genes of circulating mononuclear cells in vivo in healthy humans. We also studied the variations of the LDL receptor-related protein (LRP) gene in the same conditions. Dieters (n = 5) were submitted to a 4-d fat restriction (mean cholesterol intake: 6+/-4 mg/d), followed by a 7-d cholesterol (a mean of 791+/-150 mg/d) supplementation. Controls (n = 3) did not change their diet. During fat restriction, serum total and LDL cholesterol decreased significantly (P &lt; 0.05), and LDL receptor and HMG-CoA reductase mRNA copy numbers in mononuclear cells increased by 57 and 147%, respectively (P &lt; 0.05). After reintroducing cholesterol, serum cholesterol was stable whereas LDL receptor and HMG-CoA reductase mRNA decreased by 46 and 72% (P &lt; 0.05) and LRP mRNA increased by 59% (P &lt; 0.005). The changes in LDL receptor and HMG-CoA reductase mRNA abundance were correlated (r = +0.79, P = 0.02) during cholesterol reintroduction as were LDL receptor and LRP mRNA levels, but negatively (r = -0.70, P = 0.05). Also, 70% of the variability in LRP mRNA (P &lt; 0.005) was explained by dietary cholesterol. Thus, the basic mechanisms regulating cellular cholesterol content, the coordinate feedback repression of genes governing the synthesis and uptake of cholesterol, are operating in vivo in humans. However, serum cholesterol did not increase in response to dietary cholesterol, suggesting that these mechanisms may not play as predominant a role as previously believed in the short-term control of serum cholesterol in vivo in humans. A new finding is that LRP gene is also sensitive to dietary cholesterol, suggesting that it may participate in the control of serum cholesterol. Further in vivo studies in humans are warranted to explore the molecular mechanisms of the physiological response to dietary cholesterol in humans.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-707" status="OLD">
          <idno type="IdRef">14728936X</idno>
          <idno type="ISNI">0000 0004 4687 1979</idno>
          <idno type="RNSR">199511969L</idno>
          <idno type="ROR">https://ror.org/03985kf35</idno>
          <orgName>Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525</orgName>
          <orgName type="acronym">TIMC-IMAG</orgName>
          <date type="start">1993-01-01</date>
          <date type="end">2015-12-31</date>
          <desc>
            <address>
              <addrLine>Domaine de la Merci, 38706 La Tronche, France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www-timc.imag.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-51016" type="direct"/>
            <relation active="#struct-89889" type="direct"/>
            <relation active="#struct-301767" type="direct"/>
            <relation name="UMR5525" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="researchteam" xml:id="struct-40056" status="VALID">
          <orgName>Laboratoire d'Etudes du Comportement à Long Terme des matériaux de conditionnement</orgName>
          <orgName type="acronym">LCLT</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation name="DEN/DE2D" active="#struct-493924" type="direct"/>
            <relation active="#struct-1007585" type="indirect"/>
            <relation name="CEA/DES" active="#struct-300016" type="indirect"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-51016" status="OLD">
          <idno type="IdRef">026404796</idno>
          <idno type="ROR">https://ror.org/02aj0kh94</idno>
          <orgName>Université Joseph Fourier - Grenoble 1</orgName>
          <orgName type="acronym">UJF</orgName>
          <date type="end">2015-12-31</date>
          <desc>
            <address>
              <addrLine>BP 53 - 38041 Grenoble Cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ujf-grenoble.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-89889" status="OLD">
          <idno type="IdRef">026388804</idno>
          <idno type="ROR">https://ror.org/05sbt2524</idno>
          <orgName>Institut polytechnique de Grenoble - Grenoble Institute of Technology</orgName>
          <orgName type="acronym">Grenoble INP</orgName>
          <date type="start">2007-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>46 avenue Félix Viallet 38031 Grenoble Cedex 1</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.grenoble-inp.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-301767" status="VALID">
          <idno type="ROR">https://ror.org/01c7wz417</idno>
          <orgName>VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement</orgName>
          <orgName type="acronym">VAS</orgName>
          <date type="start">2010-01-01</date>
          <desc>
            <address>
              <addrLine>Université de Lyon, VetAgro Sup, 69280 Marcy l'Etoile (campus vétérinaire); Université de Clermont, VetAgro Sup, 63370 Lempdes (campus agronomique)</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.vetagro-sup.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="department" xml:id="struct-493924" status="VALID">
          <idno type="IdRef">22709560X</idno>
          <idno type="RNSR">201722545L</idno>
          <orgName>Département de recherche sur les Procédés et Matériaux pour les Environnements complexes (ex-DE2D)</orgName>
          <orgName type="acronym">DPME</orgName>
          <date type="start">2017-01-01</date>
          <desc>
            <address>
              <addrLine>(ex-DE2D Département de recherche sur les technologies pour l'enrichissement, le démantèlement et les déchets)(ex-DTCD et DTEC)CEA - Centre de MarcouleB.P. 1717130207, Bagnols-sur-Cèze Cedex</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-1007585" type="direct"/>
            <relation name="CEA/DES" active="#struct-300016" type="indirect"/>
          </listRelation>
        </org>
        <org type="regrouplaboratory" xml:id="struct-1007585" status="VALID">
          <idno type="RNSR">202023588G</idno>
          <orgName>Institut des Sciences et technologies pour une Economie Circulaire des énergies bas carbone</orgName>
          <orgName type="acronym">ISEC</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>CEA Marcoule</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation name="CEA/DES" active="#struct-300016" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300016" status="VALID">
          <idno type="IdRef">026372061</idno>
          <idno type="ISNI">0000000122998025</idno>
          <idno type="ROR">https://ror.org/00jjx8s55</idno>
          <idno type="Wikidata">Q868550</idno>
          <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName>
          <orgName type="acronym">CEA</orgName>
          <desc>
            <address>
              <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cea.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>