The bioaccumulation and depuration kinetics of selected metals (Ag, Co, Hg, Mn and Zn) were determined in the European variegated scallop Chlamys varia following exposures via seawater and food, using highly sensitive radiotracer techniques (110mAg, 57Co, 203Hg, 54Mn and 65Zn). Body distribution of Ag, Co, Mn and Zn was similar for both waterborne and dietary metals. Ag was mainly present in the digestive gland (> 80%); Co and Mn were generally localized in similar proportions in the digestive gland and kidneys, whereas Zn was mainly found in the kidneys (> 40%). In contrast, Hg was mainly present in gills during seawater exposure whereas it was mainly distributed in the digestive gland after food exposure. The results from all the experiments have been integrated into a bioaccumulation model in order to delineate the relative contribution of each metal uptake route in C. varia. Computation indicated that food is the main uptake route of Ag, Co, Mn, and Zn in scallop, whereas waterborne and dietary pathways were shown to contribute similarly in the global bioaccumulation of Hg in C. varia. Except for Hg, dietary transfer was investigated using two different phytoplankton species (Isochrysis galbana and Skeletonema costatum). For Mn and Zn, the dietary contribution was not influenced by the phytoplankton species used. In contrast, food quality played a major role for Ag and Co intake, (e.g., dietary pathway contributed for 97% of global Ag bioaccumulation when S. costatum was used as food vs. 58% for Ag ingested with I. galbana).