The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32-A resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values. - Archive ouverte HAL
Article Dans Une Revue Journal of Biological Inorganic Chemistry Année : 2006

The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32-A resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values.

Petros Giastas
  • Fonction : Auteur
Nikos Pinotsis
  • Fonction : Auteur
Georgios Efthymiou
  • Fonction : Auteur
Matthias Wilmanns
  • Fonction : Auteur
Panayotis Kyritsis
  • Fonction : Auteur
Irene M Mavridis
  • Fonction : Auteur

Résumé

The structure of the 2[4Fe-4S] ferredoxin (PaFd) from Pseudomonas aeruginosa, which belongs to the Allochromatium vinosum (Alvin) subfamily, has been determined by X-ray crystallography at 1.32-A resolution, which is the highest up to now for a member of this subfamily of Fds. The main structural features of PaFd are similar to those of AlvinFd. However, the significantly higher resolution of the PaFd structure makes possible a reliable comparison with available high-resolution structures of [4Fe-4S]-containing Fds, in an effort to rationalize the unusual electrochemical properties of Alvin-like Fds. Three major factors contributing to the reduction potential values of [4Fe-4S]2+/+ clusters of Fds, namely, the surface accessibility of the clusters, the N-H...S hydrogen-bonding network, and the volume of the cavities hosting the clusters, are extensively discussed. The volume of the cavities is introduced in the present work for the first time, and can in part explain the very negative potential of cluster I of Alvin-like Fds.

Dates et versions

hal-00382023 , version 1 (07-05-2009)

Identifiants

Citer

Petros Giastas, Nikos Pinotsis, Georgios Efthymiou, Matthias Wilmanns, Panayotis Kyritsis, et al.. The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32-A resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values.. Journal of Biological Inorganic Chemistry, 2006, 11 (4), pp.445-58. ⟨10.1007/s00775-006-0094-9⟩. ⟨hal-00382023⟩
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