Validation of ten new polymorphic tandem repeat loci and application to the MLVA typing of Burkholderia pseudomallei isolates collected in Singapore from 1988 to 2004. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Microbiological Methods Année : 2009

Validation of ten new polymorphic tandem repeat loci and application to the MLVA typing of Burkholderia pseudomallei isolates collected in Singapore from 1988 to 2004.

Michelle Wong Su Yen
  • Fonction : Auteur
Olivier Lisanti
  • Fonction : Auteur
François Thibault
  • Fonction : Auteur
Toh Su San
  • Fonction : Auteur
Loh Gek Kee
  • Fonction : Auteur
Valérie Hilaire
  • Fonction : Auteur
Lim Jiali
  • Fonction : Auteur
Heinrich Neubauer
  • Fonction : Auteur
Vincent Ramisse
  • Fonction : Auteur

Résumé

Multiplelocus variablenumber of tandem repeat (VNTR) analysis (MLVA) has been shown to be very promising for the typing of Burkholderia pseudomallei and mallei. The currently available set of loci requires high resolution allele size measurement due to short repeat units. The present work was aimed at expanding the available set of VNTR loci, and generating data from a collection of 102 B. pseudomallei strains isolated in Singapore between 1988 and 2004 including few additional strains of various origins as references. Ten new VNTRs with a longer array size have been identified compatible with standard agarose gel separation, and a reference database of 72 genotypes was created which can be queried on the Internet.

Dates et versions

hal-00374726 , version 1 (09-04-2009)

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Citer

Michelle Wong Su Yen, Olivier Lisanti, François Thibault, Toh Su San, Loh Gek Kee, et al.. Validation of ten new polymorphic tandem repeat loci and application to the MLVA typing of Burkholderia pseudomallei isolates collected in Singapore from 1988 to 2004.. Journal of Microbiological Methods, 2009, epub ahead of print. ⟨10.1016/j.mimet.2009.03.005⟩. ⟨hal-00374726⟩
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