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Article Dans Une Revue Applied and Environmental Microbiology Année : 2009

Characterization of Denitrification Gene Clusters of Soil Bacteria via a Metagenomic Approach

Sandrine Demanèche
  • Fonction : Auteur
Maude M. David
  • Fonction : Auteur
Elisabeth Navarro
Timothy M Vogel
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Pascal Simonet

Résumé

We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.

Dates et versions

hal-00368298 , version 1 (16-03-2009)

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Citer

Sandrine Demanèche, Laurent Philippot, Maude M. David, Elisabeth Navarro, Timothy M Vogel, et al.. Characterization of Denitrification Gene Clusters of Soil Bacteria via a Metagenomic Approach. Applied and Environmental Microbiology, 2009, 75 (2), pp.534-537. ⟨10.1128/aem.01706-08⟩. ⟨hal-00368298⟩
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