Peroxisomal beta-oxidation activities and gamma-decalactone production by the yeast Yarrowia lipolytica. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Applied Microbiology and Biotechnology Année : 1998

Peroxisomal beta-oxidation activities and gamma-decalactone production by the yeast Yarrowia lipolytica.

Résumé

gamma-Decalactone is a peachy aroma compound resulting from the peroxisomal beta-oxidation of ricinoleic acid by yeasts. The expression levels of acyl-CoA oxidase (gene deletion) and 3-ketoacyl-CoA thiolase activities (gene amplification on replicative plasmids) were modified in the yeast Yarrowia lipolytica. The effects of these modifications on beta-oxidation were measured. Overexpression of thiolase activity did not have any effect on the overall beta-oxidation activity. The disruption of one of the acyl-CoA oxidase genes resulted in an enhanced activity. The enhancement led to an increase of overall beta-oxidation activity but reduced the gamma-decalactone production rates. This seemed to indicate a non-rate-limiting role for beta-oxidation in the biotransformation of ricinoleic acid to gamma-decalactone by the yeast Yarrowia lipolytica. All strains produced and then consumed gamma-decalactone. We checked the ability of the different strains to consume gamma-decalactone in a medium containing the lactone as sole carbon source. The consumption of the strain overexpressing acyl-CoA oxidase activity was higher than that of the wild-type strain. We concluded that peroxisomal beta-oxidation is certainly involved in gamma-decalactone catabolism by the yeast Y. lipolytica. The observed production rates probably depend on an equilibrium between production and consumption of the lactone.
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hal-00368241 , version 1 (15-03-2009)

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Y. Pagot, A. Le Clainche, J. M. Nicaud, Yves Waché, J. M. Belin. Peroxisomal beta-oxidation activities and gamma-decalactone production by the yeast Yarrowia lipolytica.. Applied Microbiology and Biotechnology, 1998, 49 (3), pp.295-300. ⟨hal-00368241⟩
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