Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: a guide for prokaryotic and eukaryotic investigation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biotechnology Journal Année : 2008

Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: a guide for prokaryotic and eukaryotic investigation.

Résumé

Many fluorescent techniques are employed to evaluate the viability and activity of microbial cells used in biotechnology. These techniques are sometimes complex and the interpretation of results opened to misunderstanding. Moreover, new developments are constantly proposed especially concerning a more accurate evaluation of the state of the cells including eukaryotic microorganisms. This paper aims at presenting to biotechnologists unfamiliar with fluorescence the principles of these methods and the related possible pitfalls. It focuses on probes of the physical (integrity and fluidity) and energetical (intracellular pH and membrane potential) state of the cell membrane (bacterial and yeast cells) and presents also other probes (nucleic acids, respiration...) and new technical trends. The specificities of Gram-negative bacterial cells are also discussed.

Domaines

Biotechnologies

Dates et versions

hal-00368183 , version 1 (14-03-2009)

Identifiants

Citer

Lan Cao-Hoang, Pierre-André Marechal, Mai Lê-Thanh, Patrick Gervais, Yves Waché. Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: a guide for prokaryotic and eukaryotic investigation.. Biotechnology Journal, 2008, 3 (7), pp.890-903. ⟨10.1002/biot.200700206⟩. ⟨hal-00368183⟩

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