Preparative scale enzymatic synthesis of D-sedoheptulose-7-phosphate from β-hydroxypyruvate and D-ribose-5-phosphate

An enzymatic method for ready access to D-sedoheptulose-7-phosphate on a preparative scale was developed, based on the irreversible transketolase-catalyzed reaction: β-hydroxypyruvate + D-ribose-5- phosphate → D-sedoheptulose-7-phosphate. D-Sedoheptulose-7-phosphate disodium salt was obtained in 81% overall yield determined using a standard curve obtained by LC/MS/MS.

Mots clés

D-Sedoheptulose-7-phosphate Biocatalysis Transketolase

Domaines

Catalyse

Fichier principal

J_Mol_Cat_B_Enz.pdf (262.03 Ko)

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Stéphanie Bonnefoy : Connectez-vous pour contacter le contributeur

https://hal.science/hal-00366191

Soumis le : vendredi 6 mars 2009-10:17:10

Dernière modification le : vendredi 31 mai 2024-17:08:16

Archivage à long terme le : mardi 8 juin 2010-21:20:52

Dates et versions

hal-00366191 , version 1 (06-03-2009)

Identifiants

HAL Id : hal-00366191 , version 1

Citer

Franck Charmantray, Virgil Hélaine, Bertrand Legeret, Laurence Hecquet. Preparative scale enzymatic synthesis of D-sedoheptulose-7-phosphate from β-hydroxypyruvate and D-ribose-5-phosphate. Journal of Molecular Catalysis B: Enzymatic, 2009, 57, pp.6-9. ⟨hal-00366191⟩

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