Cell wall component and mycotoxin moieties involved in binding of fumonisin B1 and B2 by lactic acid bacteria. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Applied Microbiology Année : 2009

Cell wall component and mycotoxin moieties involved in binding of fumonisin B1 and B2 by lactic acid bacteria.

Résumé

Aims: The ability of lactic acid bacteria (LAB) to bind fumonisins B1 and B2 (FB1, FB2) in fermented foods and feeds and in the gastrointestinal tract could contribute to decrease their bioavailability and toxic effects on farm animals and humans. The aim of this work was to identify the bacterial cell wall component(s) and the functional group(s) of FB involved in the LAB-FB interaction. Methods and Results: The effect of physicochemical, enzymatic and genetic treatments of bacteria and the removal/inactivation of the functional groups of FB on toxin binding were evaluated. Treatments affecting the bacterial wall polysaccharides, lipids and proteins increased binding, while those degrading peptidoglycan (PG) partially decreased it. In addition, purified PG from Gram-positive bacteria bound FB in a manner analogue to that of intact LAB. For FB, tricarballylic acid (TCA) chains play a significant role in binding as hydrolysed FB had less affinity for LAB. Conclusions: Peptidoglycan and TCA are important components of LAB and FB, respectively, involved in the binding interaction. Significance and Impact of the Study: Lactic acid bacteria binding efficiency seems related to the peptide moiety structure of the PG. This information can be used to select probiotics with increased FB binding efficiency.
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Dates et versions

hal-00360818 , version 1 (12-02-2009)
hal-00360818 , version 2 (09-03-2009)

Identifiants

  • HAL Id : hal-00360818 , version 2

Citer

Vincent Niderkorn, Diego Morgavi, Bettina Aboab, Marielle Lemaire, Hamid Boudra. Cell wall component and mycotoxin moieties involved in binding of fumonisin B1 and B2 by lactic acid bacteria.. Journal of Applied Microbiology, 2009, 106, pp.977-985. ⟨hal-00360818v2⟩
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