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Article Dans Une Revue RNA Année : 2008

Toward predicting self-splicing and protein-facilitated splicing of group I introns.

Résumé

In the current era of massive discoveries of noncoding RNAs within genomes, being able to infer a function from a nucleotide sequence is of paramount interest. Although studies of individual group I introns have identified self-splicing and nonself-splicing examples, there is no overall understanding of the prevalence of self-splicing or the factors that determine it among the >2300 group I introns sequenced to date. Here, the self-splicing activities of 12 group I introns from various organisms were assayed under six reaction conditions that had been shown previously to promote RNA catalysis for different RNAs. Besides revealing that assessing self-splicing under only one condition can be misleading, this survey emphasizes that in vitro self-splicing efficiency is correlated with the GC content of the intron (>35% GC was generally conductive to self-splicing), and with the ability of the introns to form particular tertiary interactions. Addition of the Neurospora crassa CYT-18 protein activated splicing of two nonself-splicing introns, but inhibited the second step of self-splicing for two others. Together, correlations between sequence, predicted structure and splicing begin to establish rules that should facilitate our ability to predict the self-splicing activity of any group I intron from its sequence.

Dates et versions

hal-00356170 , version 1 (26-01-2009)

Identifiants

Citer

Quentin Vicens, Paul J Paukstelis, Eric Westhof, Alan M Lambowitz, Thomas R Cech. Toward predicting self-splicing and protein-facilitated splicing of group I introns.. RNA, 2008, 14 (10), pp.2013-29. ⟨10.1261/rna.1027208⟩. ⟨hal-00356170⟩

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