Contribution of energy restriction and macronutrient composition to changes in adipose tissue gene expression during dietary weight-loss programs in obese women.
Frédéric Capel
(1, 2, 3, 4, 5)
,
Nathalie Viguerie
(1, 2, 4, 5)
,
Nathalie Vega
(1, 3, 6, 7, 8)
,
Sébastien Dejean
(9)
,
Peter Arner
(10)
,
Eva Klimcáková
(1, 2, 3, 4, 5, 11, 12)
,
J Alfredo Martinez
(11, 12)
,
Wim H. M. Saris
(13)
,
Claus Holst
(14)
,
Moira A. Taylor
(15)
,
Jean-Michel Oppert
(16, 17)
,
Thorkild I. A. Sørensen
(14)
,
Karine Clément
(16, 17)
,
Hubert Vidal
(6, 7, 8)
,
Dominique Langin
(1, 2, 3, 4, 5)
1
I2MR -
Institut de médecine moléculaire de Rangueil
2 Service Endocrinologie, maladies métaboliques et nutrition [CHU Toulouse]
3 IFR 31 - IFR 31 Louis Bugnard
4 Service Anatomie et cytologie pathologiques [CHU Toulouse]
5 Franco-Czech Laboratory for Clinical Research on Obesity
6 Faculté de Médecine Lyon-Sud
7 Centre de Recherche en Nutrition Humaine
8 RMND UM55 - Régulations métaboliques, nutrition et diabètes - UM55
9 IMT - Institut de Mathématiques de Toulouse UMR5219
10 Department of Midicine Karolinska Institute
11 Department of Sports Medicine of Charles University
12 Department of Physiology and nutrition University of Navarra
13 Department of Human Biology Nutrition and Toxicology Research Institute
14 The Netherlands Institute of Preventive Medicine
15 School of Biomedical Sciences Queen's Medical Center
16 CRC (UMR_S 872) - Centre de Recherche des Cordeliers
17 CHU Pitié-Salpêtrière [AP-HP]
2 Service Endocrinologie, maladies métaboliques et nutrition [CHU Toulouse]
3 IFR 31 - IFR 31 Louis Bugnard
4 Service Anatomie et cytologie pathologiques [CHU Toulouse]
5 Franco-Czech Laboratory for Clinical Research on Obesity
6 Faculté de Médecine Lyon-Sud
7 Centre de Recherche en Nutrition Humaine
8 RMND UM55 - Régulations métaboliques, nutrition et diabètes - UM55
9 IMT - Institut de Mathématiques de Toulouse UMR5219
10 Department of Midicine Karolinska Institute
11 Department of Sports Medicine of Charles University
12 Department of Physiology and nutrition University of Navarra
13 Department of Human Biology Nutrition and Toxicology Research Institute
14 The Netherlands Institute of Preventive Medicine
15 School of Biomedical Sciences Queen's Medical Center
16 CRC (UMR_S 872) - Centre de Recherche des Cordeliers
17 CHU Pitié-Salpêtrière [AP-HP]
Frédéric Capel
- Fonction : Auteur
- PersonId : 736303
- IdHAL : frederic-capel
- ORCID : 0000-0002-0133-0277
- IdRef : 084732369
Nathalie Viguerie
- Fonction : Auteur
- PersonId : 862472
- ORCID : 0000-0002-1730-9915
- IdRef : 121171620
Sébastien Dejean
- Fonction : Auteur
- PersonId : 17534
- IdHAL : sebastien-dejean
- ORCID : 0000-0001-9610-5306
- IdRef : 06889564X
Eva Klimcáková
- Fonction : Auteur
Karine Clément
- Fonction : Auteur
- PersonId : 756892
- ORCID : 0000-0003-4619-6785
- IdRef : 07341512X
Hubert Vidal
- Fonction : Auteur
- PersonId : 21591
- IdHAL : hubert-vidal
- ORCID : 0000-0002-9467-0317
- IdRef : 034238875
Dominique Langin
- Fonction : Auteur
- PersonId : 175745
- IdHAL : dominique-langin
- ORCID : 0000-0002-2669-7825
- IdRef : 050475509
Résumé
CONTEXT: Hypoenergetic diets are used to reduce body fat mass and metabolic risk factors in obese subjects. The molecular changes in adipose tissue associated with weight loss and specifically related to the dietary composition are poorly understood. OBJECTIVE: We investigated adipose tissue gene expression from human obese women according to energy deficit and the fat and carbohydrate content of the diet. DESIGN AND SETTING: Obese subjects recruited among eight European clinical centers were followed up 10 wk of either a low-fat (high carbohydrate) or a moderate-fat (low carbohydrate) hypoenergetic diet. SUBJECTS: Two sets of 47 women in each dietary arm were selected among 648 subjects matched for anthropometric and biological parameters. MAIN OUTCOME MEASURE: We measured adipose tissue gene expression changes in one set using a candidate gene approach. The other set was used to survey 24,469 transcripts using DNA microarrays. Results were analyzed using dedicated statistical methods. Diet-sensitive regulations were confirmed on the other set of subjects. RESULTS: The two diets induced similar weight loss and similar changes for most of the biological variables except for components of the blood lipid profile. One thousand genes were regulated by energy restriction. We validated an effect of the fat to carbohydrate ratio for five genes (FABP4, NR3C1, SIRT3, FNTA, and GABARAPL2) with increased expression during the moderate-fat diet. CONCLUSIONS: Energy restriction had a more pronounced impact on variations in human adipose tissue gene expression than macronutrient composition. The macronutrient-sensitive regulation of a subset of genes may influence adipose tissue function and metabolic response.
Domaines
Endocrinologie et métabolismeFormat du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Résumé |
en
CONTEXT: Hypoenergetic diets are used to reduce body fat mass and metabolic risk factors in obese subjects. The molecular changes in adipose tissue associated with weight loss and specifically related to the dietary composition are poorly understood. OBJECTIVE: We investigated adipose tissue gene expression from human obese women according to energy deficit and the fat and carbohydrate content of the diet. DESIGN AND SETTING: Obese subjects recruited among eight European clinical centers were followed up 10 wk of either a low-fat (high carbohydrate) or a moderate-fat (low carbohydrate) hypoenergetic diet. SUBJECTS: Two sets of 47 women in each dietary arm were selected among 648 subjects matched for anthropometric and biological parameters. MAIN OUTCOME MEASURE: We measured adipose tissue gene expression changes in one set using a candidate gene approach. The other set was used to survey 24,469 transcripts using DNA microarrays. Results were analyzed using dedicated statistical methods. Diet-sensitive regulations were confirmed on the other set of subjects. RESULTS: The two diets induced similar weight loss and similar changes for most of the biological variables except for components of the blood lipid profile. One thousand genes were regulated by energy restriction. We validated an effect of the fat to carbohydrate ratio for five genes (FABP4, NR3C1, SIRT3, FNTA, and GABARAPL2) with increased expression during the moderate-fat diet. CONCLUSIONS: Energy restriction had a more pronounced impact on variations in human adipose tissue gene expression than macronutrient composition. The macronutrient-sensitive regulation of a subset of genes may influence adipose tissue function and metabolic response.
|
Titre |
en
Contribution of energy restriction and macronutrient composition to changes in adipose tissue gene expression during dietary weight-loss programs in obese women.
|
Auteur(s) |
Frédéric Capel
1, 2, 3, 4, 5
, Nathalie Viguerie
1, 2, 4, 5
, Nathalie Vega
1, 3, 6, 7, 8
, Sébastien Dejean
9
, Peter Arner
10
, Eva Klimcáková
1, 2, 3, 4, 5, 11, 12
, J Alfredo Martinez
11, 12
, Wim H. M. Saris
13
, Claus Holst
14
, Moira A. Taylor
15
, Jean-Michel Oppert
16, 17
, Thorkild I. A. Sørensen
14
, Karine Clément
16, 17
, Hubert Vidal
6, 7, 8
, Dominique Langin
1, 2, 3, 4, 5
1
I2MR -
Institut de médecine moléculaire de Rangueil
( 27489 )
- Institut Louis Bugnard 1, avenue Jean Poulhes BP 84225 31432 TOULOUSE CEDEX 4
- France
2
Service Endocrinologie, maladies métaboliques et nutrition [CHU Toulouse]
( 551425 )
- Pôle cardiovasculaire et métabolique,
31059 Toulouse cedex 9
- France
3
IFR 31 -
IFR 31 Louis Bugnard
( 1756 )
- Bâtiments L3 L1 1 Avenue Jean Poulhes 31403 TOULOUSE CEDEX 4
- France
4
Service Anatomie et cytologie pathologiques [CHU Toulouse]
( 111195 )
- Institut Universitaire du Cancer de Toulouse - Oncopole,
1, avenue Irène Joliot-Curie - 31059 Toulouse cedex 9
- France
5
Franco-Czech Laboratory for Clinical Research on Obesity
( 99000 )
- 3rd Faculty of Medicine, Prague
- Tchéquie
6
Faculté de Médecine Lyon-Sud
( 86344 )
- 69622 LYON
- France
7
Centre de Recherche en Nutrition Humaine
( 86345 )
- LYON
- France
8
RMND UM55 -
Régulations métaboliques, nutrition et diabètes - UM55
( 533233 )
- France
9
IMT -
Institut de Mathématiques de Toulouse UMR5219
( 1954 )
- UPS IMT, F-31062 Toulouse Cedex 9,
INSA Toulouse, F-31077 Toulouse,
France UT1, F-31042 Toulouse,
France UT2, F-31058 Toulouse,
Téléphone : 05.61.55.67.90
- France
10
Department of Midicine Karolinska Institute
( 86347 )
- SE-171 77 STOCKHOLM
- Suède
11
Department of Sports Medicine of Charles University
( 86348 )
- 100 34 PRAGUE
- Tchéquie
12
Department of Physiology and nutrition University of Navarra
( 86349 )
- 31080 PAMPLONA
- Espagne
13
Department of Human Biology Nutrition and Toxicology Research Institute
( 86350 )
- NL-6200 MD MAASTRICHT
- France
14
The Netherlands Institute of Preventive Medicine
( 86351 )
- Pays-Bas
15
School of Biomedical Sciences Queen's Medical Center
( 86352 )
- NG7 2UH NOTTINGHAM
- Royaume-Uni
16
CRC (UMR_S 872) -
Centre de Recherche des Cordeliers
( 27744 )
- CRBM des Cordeliers 15, rue de l'ecole de medecine batiment E 75270 Paris cedex 06
- France
17
CHU Pitié-Salpêtrière [AP-HP]
( 353778 )
- 47-83 Boulevard de l'Hôpital, 75013 Paris
- France
|
Date de publication |
2008-11
|
Date de publication électronique |
2008-09-09
|
Volume |
93
|
Numéro |
11
|
Page/Identifiant |
4315-22
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Nom de la revue |
|
Langue du document |
Anglais
|
Public visé |
Scientifique
|
Mots-clés (Mesh) |
|
Voir aussi |
|
Domaine(s) |
|
Mots-clés |
en
Adaptor Proteins, Signal Transducing, Adipose Tissue, Adult, Body Composition, Body Mass Index, Reducing, Energy Intake, Energy Metabolism, Fatty Acid-Binding Proteins, Genetic Variation, Humans, Obesity, Oligonucleotide Array Sequence Analysis, RNA, Glucocorticoid, Genetic, Weight Loss
fr
Diet, Female, Microfilament Proteins, Mitochondrial Proteins, Receptors, Sirtuins, Transcription
|
DOI | 10.1210/jc.2008-0814 |
ProdINRA | 250036 |
Pubmed Id | 18782868 |
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