<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-00353456</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-19T05:28:36+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Flavin-dependent thymidylate synthase X limits chromosomal DNA replication.</title>
            <author role="aut">
              <persName>
                <forename type="first">Frédéric</forename>
                <surname>Escartin</surname>
              </persName>
              <idno type="halauthorid">248732-0</idno>
              <affiliation ref="#struct-1361"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Stéphane</forename>
                <surname>Skouloubris</surname>
              </persName>
              <email type="md5">efa125bc50abb387b5062b12700b1a99</email>
              <email type="domain">polytechnique.edu</email>
              <idno type="idhal" notation="string">stephaneskouloubris</idno>
              <idno type="idhal" notation="numeric">1332896</idno>
              <idno type="halauthorid" notation="string">166898-1332896</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6660-0023</idno>
              <affiliation ref="#struct-1361"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Ursula</forename>
                <surname>Liebl</surname>
              </persName>
              <idno type="idhal" notation="numeric">756972</idno>
              <idno type="halauthorid" notation="string">164188-756972</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-0869-4388</idno>
              <idno type="IDREF">https://www.idref.fr/131867091</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Hannu</forename>
                <surname>Myllykallio</surname>
              </persName>
              <idno type="halauthorid">166900-0</idno>
              <affiliation ref="#struct-1361"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Isabelle</forename>
                <surname>HUE</surname>
              </persName>
              <email type="md5">e0c7f5a97ee2162a259541b971b2e5f1</email>
              <email type="domain">i2bc.paris-saclay.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2009-01-15 15:44:09</date>
              <date type="whenModified">2024-01-09 10:10:41</date>
              <date type="whenReleased">2009-01-15 15:44:09</date>
              <date type="whenProduced">2008-07-22</date>
              <ref type="externalLink" target="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2481370"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="640679">
                <persName>
                  <forename>Isabelle</forename>
                  <surname>HUE</surname>
                </persName>
                <email type="md5">e0c7f5a97ee2162a259541b971b2e5f1</email>
                <email type="domain">i2bc.paris-saclay.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-00353456</idno>
            <idno type="halUri">https://hal.science/hal-00353456</idno>
            <idno type="halBibtex">escartin:hal-00353456</idno>
            <idno type="halRefHtml">&lt;i&gt;Proceedings of the National Academy of Sciences of the United States of America&lt;/i&gt;, 2008, 105 (29), pp.9948-52. &lt;a target="_blank" href="https://dx.doi.org/10.1073/pnas.0801356105"&gt;&amp;#x27E8;10.1073/pnas.0801356105&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Proceedings of the National Academy of Sciences of the United States of America, 2008, 105 (29), pp.9948-52. &amp;#x27E8;10.1073/pnas.0801356105&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-PSUD">Université Paris Sud - Paris XI</idno>
            <idno type="stamp" n="I2BC">I2BC</idno>
            <idno type="stamp" n="UNIV-PARIS-SACLAY">Université Paris-Saclay</idno>
            <idno type="stamp" n="UNIV-PSUD-SACLAY" corresp="UNIV-PARIS-SACLAY">Université Paris Sud pour Paris Saclay</idno>
            <idno type="stamp" n="GS-HEALTH-DRUG-SCIENCES">Graduate School Health and Drug Sciences</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Flavin-dependent thymidylate synthase X limits chromosomal DNA replication.</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Frédéric</forename>
                    <surname>Escartin</surname>
                  </persName>
                  <idno type="halauthorid">248732-0</idno>
                  <affiliation ref="#struct-1361"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Stéphane</forename>
                    <surname>Skouloubris</surname>
                  </persName>
                  <email type="md5">efa125bc50abb387b5062b12700b1a99</email>
                  <email type="domain">polytechnique.edu</email>
                  <idno type="idhal" notation="string">stephaneskouloubris</idno>
                  <idno type="idhal" notation="numeric">1332896</idno>
                  <idno type="halauthorid" notation="string">166898-1332896</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6660-0023</idno>
                  <affiliation ref="#struct-1361"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Ursula</forename>
                    <surname>Liebl</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">756972</idno>
                  <idno type="halauthorid" notation="string">164188-756972</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-0869-4388</idno>
                  <idno type="IDREF">https://www.idref.fr/131867091</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Hannu</forename>
                    <surname>Myllykallio</surname>
                  </persName>
                  <idno type="halauthorid">166900-0</idno>
                  <affiliation ref="#struct-1361"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">7969</idno>
                <idno type="issn">0027-8424</idno>
                <idno type="eissn">1091-6490</idno>
                <title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
                <imprint>
                  <publisher>National Academy of Sciences</publisher>
                  <biblScope unit="volume">105</biblScope>
                  <biblScope unit="issue">29</biblScope>
                  <biblScope unit="pp">9948-52</biblScope>
                  <date type="datePub">2008-07-22</date>
                  <date type="dateEpub">2008-07-11</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1073/pnas.0801356105</idno>
              <idno type="pubmed">18621705</idno>
              <idno type="pubmedcentral">PMC2481370</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="mesh">Catalysis</classCode>
              <classCode scheme="mesh">Chromosomes, Bacterial</classCode>
              <classCode scheme="mesh">Lactobacillus casei</classCode>
              <classCode scheme="mesh">Recombinant Proteins</classCode>
              <classCode scheme="mesh">Thymidylate Synthase</classCode>
              <classCode scheme="mesh">DNA Replication</classCode>
              <classCode scheme="mesh">DNA, Bacterial</classCode>
              <classCode scheme="mesh">Escherichia coli</classCode>
              <classCode scheme="mesh">Flavin-Adenine Dinucleotide</classCode>
              <classCode scheme="mesh">Gene Deletion</classCode>
              <classCode scheme="mesh">Genes, Bacterial</classCode>
              <classCode scheme="mesh">Helicobacter pylori</classCode>
              <classCode scheme="mesh">Kinetics</classCode>
              <classCode scheme="halDomain" n="sdv.bbm">Life Sciences [q-bio]/Biochemistry, Molecular Biology</classCode>
              <classCode scheme="halDomain" n="sdv.mp">Life Sciences [q-bio]/Microbiology and Parasitology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>We have investigated the hitherto unexplored possibility that differences in the catalytic efficiencies of thymidylate synthases ThyX and ThyA, enzymes that produce the essential DNA precursor dTMP, have influenced prokaryotic genome evolution. We demonstrate that DNA replication speed in bacteria and archaea that contain the low-activity ThyX enzyme is up to 10-fold decreased compared with species that contain the catalytically more efficient ThyA. Our statistical studies of &gt;400 genomes indicated that ThyA proteins are preferred for the replication of large genomes, providing further evidence that the thymidylate metabolism is limiting expansion of prokaryotic genomes. Because both ThyX and ThyA participate in frequent reciprocal gene replacement events, our observations indicate that the bacterial metabolism continues to modulate the size and composition of prokaryotic genomes. We also propose that the increased kinetic efficiency of thymidylate synthesis has contributed to extending the prokaryotic evolutionary potential.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-1361" status="OLD">
          <orgName>Institut de génétique et microbiologie [Orsay]</orgName>
          <orgName type="acronym">IGM</orgName>
          <date type="end">2014-12-31</date>
          <desc>
            <address>
              <addrLine>Bâtiments 400 et 409UFR des Sciences - Université Paris-Sud 1115, rue Georges Clémenceau - F 91405 ORSAY Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.igmors.u-psud.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-92966" type="direct"/>
            <relation name="UMR8621" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-92966" status="OLD">
          <idno type="ROR">https://ror.org/028rypz17</idno>
          <orgName>Université Paris-Sud - Paris 11</orgName>
          <orgName type="acronym">UP11</orgName>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.u-psud.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>