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Communication Dans Un Congrès Année : 2004

Radiofrequency radiations emitted by mobile phones and the brain: an in vivo approach

Résumé

In the context of the concerns and fears over mobile telephony and health, various research programmes have been launched worldwide. Several of our research projects deal with molecular biology that uses techniques investigating biological effects at the DNA, protein and cellular level. Among them are cytogenetics, gene and protein expressions that can be related to cancer and can be studied using in vivo models. DNA damage and Hsp70 expression in the brain of rats have been investigated after exposure to the GSM-900 signal used for mobile telephony. The head of the rats was only exposed using an antenna operating at 900 MHz. After a one-week training period, rats were exposed (antenna ON) or sham-exposed (antenna OFF) to GSM-900 in individual rockets. Free moving rats were used as cage-controls. (i) The DNA damage caused by GSM-900 microwaves in brain cells at different SAR was assessed using the alkaline comet assay method. Male Sprague-Dawley rats were divided into 6 groups: cage control (n=5), positive control (n=8) (25 mg/kg of ethylmethane sulfonate, i. p. ), sham-exposed (n=8) and exposed animals (n=8) to 1, 5; 3 or 6 W/kg local SAR for 2 hours and killed by decapitation immediately after exposure. A tissue press was used to obtain a single cell suspension from the whole brain. No consistent difference in the amount of damaged cells could be observed whatever the SAR used. (ii) The expression of Hsp70 after a one-hour exposure to GSM-900 signal was assessed in male Wistar rats. The animals were divided in four groups: positive controls (kainic acid injected) (n=2), cage-controls (n=4), sham-exposed (n=5) and GSM-900 (2 W/kg) exposed rats (n=5). Immediately after exposure, brains were perfused with paraformaldehyde solution (4% in PBS) by intracardiac pathway and frozen. Brains are currently under analysis for Hsp70 expression by immunohistochemistry. The results will be presented at the meeting.
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Dates et versions

hal-00335848 , version 1 (30-10-2008)

Identifiants

  • HAL Id : hal-00335848 , version 1

Citer

I. Lagroye, F. Poulletier de Gannes, E. Haro, M. Taxile, R. Anane, et al.. Radiofrequency radiations emitted by mobile phones and the brain: an in vivo approach. 4th Forum of European NeuroScience, Jul 2004, Lisbonne, Portugal. ⟨hal-00335848⟩
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