A near-infrared diode laser spectrometer was used in the laboratory to study CO2 line parameters near 2.05 μm. The spectral region was studied with a new generation of laser: antimonide-based quantum-well diode laser from University of Montpellier––France. One line of the (2 00 1)III ← (0 0 0) band of CO2 that is suitable for the in situ sensing of the middle atmosphere has been thoroughly studied at 2.05 μm. The results are compared to previous determinations and available databases. We further demonstrate with this new-generation laser the possibility to detect CO2 at ground levels using the JETDLAG laboratory spectrometer.