A new genetic locus controlling growth and proliferation in Drosophila melanogaster. - Archive ouverte HAL Access content directly
Journal Articles Genetics Year : 2003

A new genetic locus controlling growth and proliferation in Drosophila melanogaster.

Abstract

Multicellular organisms grow through both proliferation and growth of their individual cells. We have conducted a P-element-based misexpression screen for genes whose upregulation alters wing disc growth during development. One particular group of four P elements, all inserted at cytological location 61C7-8, exhibited specific overgrowth upon misexpression in proliferating imaginal tissues. Clonal analysis revealed that upon misexpression, cell number was increased but cell size was not affected, indicating that cell growth and proliferation were induced in a coordinate manner. Loss of function at the locus produced small flies with reduced cell number, consistent with the presence of a gene encoding a positive growth regulator. We characterized a new transcription unit initiating in a region adjacent to the P insertions, which generated a complex series of polyadenylated transcripts. Although these RNAs were induced in response to misexpression, none was sufficient by itself to recapitulate overgrowth when overexpressed. This suggested either that a particular combination of these transcripts was necessary or that other sequences are involved.
No file

Dates and versions

hal-00322347 , version 1 (17-09-2008)

Identifiers

  • HAL Id : hal-00322347 , version 1
  • PUBMED : 12871911

Cite

Sophie Raisin, Sophie Pantalacci, Jean-Philippe Breittmayer, Pierre Léopold. A new genetic locus controlling growth and proliferation in Drosophila melanogaster.. Genetics, 2003, 164 (3), pp.1015-25. ⟨hal-00322347⟩
42 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More