Different microtubule motors move early and late endocytic compartments - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Traffic Année : 2008

Different microtubule motors move early and late endocytic compartments

Résumé

Important progress has been made during the past decade in the identification of molecular motors required in the distribution of early and late endosomes and the proper trafficking along the endocytic pathway. There is little direct evidence, however, that these motors drive movement of the endosomes. To evaluate the contributions of kinesin-1, dynein and kinesin-2 to the movement of early and late endosomes along microtubules, we made use of a cytosol-free motility assay using magnetically isolated early and late endosomes as well as biochemical analyses and live-cell imaging. By making use of specific antibodies, we confirmed that kinesin-1 and dynein move early endosomes and we found that kinesin-2 moves both early and late endosomes in the cell-free assay. Unexpectedly, dynein did not move late endosomes in the cell-free assay. We provide evidence from disruption of dynein function and latrunculin A treatment, suggesting that dynein regulates late endosome movement indirectly, possibly through a mechanism involving the actin cytoskeleton. These data provide new insights into the complex regulation of endosomes’ motility and suggest that dynein is not the major motor required to move late endosomes toward the minus end of microtubules.
Fichier non déposé

Dates et versions

hal-00315465 , version 1 (28-08-2008)

Licence

Paternité

Identifiants

Citer

Sylvain Loubéry, Claire Wilhelm, Ilse Hurbain, Sophie Neveu, Daniel Louvard, et al.. Different microtubule motors move early and late endocytic compartments. Traffic, 2008, 9 (4), pp.492-509. ⟨10.1111/j.1600-0854.2008.00704.x⟩. ⟨hal-00315465⟩
23 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More