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Conference Papers Year : 2008

Automated cell characterization platform: Application to yeast protoplast study by electrorotation

Julie Laforet
  • Function : Correspondent author
  • PersonId : 852952

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Marie Frénéa-Robin
François Buret
Laurent Nicolas

Abstract

This paper is about the development of a new automated platform dedicated to cell manipulation and characterization by dielectrophoretic methods. We illustrate its possibilities by studying yeast protoplasts and yeast cells electrorotation spectra, obtained using polynomial microelectrode structures powered by computer-controlled generators. Measurements were made over the frequency range 100 kHz to 80MHz, mostly in a suspending medium of conductivity 50 mS/m inside the rotation chamber. The rotation rate of yeast protoplasts was inferior to that of whole yeast cells. To understand such behavioral differences, yeast protoplasts were modelled as single-shell spheres in a first approach.

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Electric power
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Dates and versions

hal-00312909 , version 1 (26-08-2008)

Identifiers

  • HAL Id : hal-00312909 , version 1
  • PRODINRA : 249867

Cite

Julie Laforet, Marie Frénéa-Robin, Hélène Cérémonie, François Buret, Laurent Nicolas. Automated cell characterization platform: Application to yeast protoplast study by electrorotation. BIODEVICES 2008, Jan 2008, Funchal (Madeira), Portugal. pp.190-193. ⟨hal-00312909⟩
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