A new 2CTAB/PCI method improves DNA amplification success from faeces of Mediterranean (Barbary macaques) and tropical (lowland gorillas) primates. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Conservation Genetics Année : 2008

A new 2CTAB/PCI method improves DNA amplification success from faeces of Mediterranean (Barbary macaques) and tropical (lowland gorillas) primates.

Résumé

Animal genomic DNA extracts of sufficient quality to address questions about population biology or behavioural ecology can be obtained from faeces when adequate extraction procedures are used. The presence of PCR inhibitors in extract products appears generally the main factor limiting DNA amplification success. We compared DNA amplification success from faeces of a tropical primate (western lowland gorilla, Gorilla g. gorilla) and a Mediterranean primate (Barbary macaque, Macaca sylvanus) between a standardized extraction technique widely used in animals (QIAamp(R) stool kit), a technique mainly used in plant species (CTAB) and a new protocol (2CTAB/PCI). Amplification success varied from 51% to 97%, the highest success being reached with the 2CTAB/PCI protocol in both species.

Dates et versions

hal-00312864 , version 1 (26-08-2008)

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Dominique Vallet, Eric Petit, Sylvain Gatti, Florence Levréro, Nelly Ménard. A new 2CTAB/PCI method improves DNA amplification success from faeces of Mediterranean (Barbary macaques) and tropical (lowland gorillas) primates.. Conservation Genetics, 2008, 9 (3), pp.677-380. ⟨10.1007/s10592-007-9361-8⟩. ⟨hal-00312864⟩
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