Origin and evolution of GALA-LRR, a new member of the CC-LRR subfamily: from plants to bacteria? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2008

Origin and evolution of GALA-LRR, a new member of the CC-LRR subfamily: from plants to bacteria?

Résumé

The phytopathogenic bacterium Ralstonia solanacearum encodes type III effectors, called GALA proteins, which contain F-box and LRR domains. The GALA LRRs do not perfectly fit any of the previously described LRR subfamilies. By applying protein sequence analysis and structural prediction, we clarify this ambiguous case of LRR classification and assign GALA-LRRs to CC-LRR subfamily. We demonstrate that side-by-side packing of LRRs in the 3D structures may control the limits of repeat variability within the LRR subfamilies during evolution. The LRR packing can be used as a criterion, complementing the repeat sequences, to classify newly identified LRR domains. Our phylogenetic analysis of F-box domains proposes the lateral gene transfer of bacterial GALA proteins from host plants. We also present an evolutionary scenario which can explain the transformation of the original plant LRRs into slightly different bacterial LRRs. The examination of the selective evolutionary pressure acting on GALA proteins suggests that the convex side of their horse-shoe shaped LRR domains is more prone to positive selection than the concave side, and we therefore hypothesize that the convex surface might be the site of protein binding relevant to the adaptor function of the F-box GALA proteins. This conclusion provides a strong background for further functional studies aimed at determining the role of these type III effectors in the virulence of R. solanacearum.
Fichier principal
Vignette du fichier
kajava_PlosONE_08.pdf (309.57 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-00292865 , version 1 (02-07-2008)

Identifiants

Citer

Andrey Kajava, Maria Anisimova, Nemo Peeters. Origin and evolution of GALA-LRR, a new member of the CC-LRR subfamily: from plants to bacteria?. PLoS ONE, 2008, 3 (2), pp.e1694. ⟨10.1371/journal.pone.0001694⟩. ⟨hal-00292865⟩
111 Consultations
644 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More