Bioluminescent imaging of Ca2+ activity reveals spatiotemporal dynamics in glial networks of dark-adapted mouse retina. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue The Journal of Physiology Année : 2007

Bioluminescent imaging of Ca2+ activity reveals spatiotemporal dynamics in glial networks of dark-adapted mouse retina.

Résumé

Glial Ca(2+) excitability plays a key role in reciprocal neuron-glia communication. In the retina, neuron-glia signalling is expected to be maximal in the dark, but the glial Ca(2+) signal characteristics under such conditions have not been evaluated. To address this question, we used bioluminescence imaging to monitor spontaneous Ca(2+) changes under dark conditions selectively in M?r cells, the principal retinal glial cells. By combining this imaging approach with network analysis, we demonstrate that activity in M?r cells is organized in networks of coactive cells, involving 2-16 cells located distantly and/or in clusters. We also report that spontaneous activity of small networks (2-6 M?r cells) repeat over time, sometimes in the same sequential order, revealing specific temporal dynamics. In addition, we show that networks of coactive glial cells are inhibited by TTX, indicating that ganglion and/or amacrine neuronal cells probably regulate M?r cell network properties. These results represent the first demonstration that spontaneous activity in adult M?r cells is patterned into correlated networks that display repeated sequences of coactivations over time. Furthermore, our bioluminescence technique provides a novel tool to study the dynamic characteristics of glial Ca(2+) events in the retina under dark conditions, which should greatly facilitate future investigations of retinal dark-adaptive processes.

Dates et versions

hal-00292267 , version 1 (30-06-2008)

Identifiants

Citer

Cendra Agulhon, Jean-Claude Platel, Bogdan Kolomiets, Valérie Forster, Serge Picaud, et al.. Bioluminescent imaging of Ca2+ activity reveals spatiotemporal dynamics in glial networks of dark-adapted mouse retina.. The Journal of Physiology, 2007, 583 (Pt 3), pp.945-58. ⟨10.1113/jphysiol.2007.135715⟩. ⟨hal-00292267⟩
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