Study of the polymorphism of metallothioneins by ion-spray mass spectrometry
Etude du polymorphisme des metallothioneines par spectrometrie de masse à source ion-spray
Résumé
Ion-spray mass spectrometry (IS-MS) is shown to be an attractive technique to identify and to differentiate among the sub-isoforms of metallothioneins (MT). Different modes of application of IS-MS are discussed using the example of MT-2 rabbit liver prepurified by size-exclusion and anion-exchange chromatography. Direct IS-MS mode, microbore HPLC with on- line IS-MS detection of fully cadmiated (Cd7-MT isoforms), HPLC with post- column acidification and detection of the apo-MT isoforms by IS-MS, and with post-column acidification and detection of the Cd ion by IS-MS are discussed.
Domaines
Chimie analytiqueOrigine | Fichiers éditeurs autorisés sur une archive ouverte |
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