Article Dans Une Revue Methods in Cell Biology Année : 2008

Chapter 2 quail-chick transplantations.

Résumé

The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.

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Dates et versions

hal-00282810 , version 1 (28-05-2008)

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Citer

Nicole Le Douarin, Françoise Dieterlen-Lièvre, Sophie Creuzet, Marie-Aimée Teillet. Chapter 2 quail-chick transplantations.. Methods in Cell Biology, 2008, 87 (4), pp.19-58. ⟨10.1016/S0091-679X(08)00202-1⟩. ⟨hal-00282810⟩
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