Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Developmental Dynamics Année : 2008

Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro.

Résumé

We have investigated whether the phenotype of myogenic clones derived from satellite cells of different muscles from the transgenic immortomouse depended on muscle type origin. Clones derived from neonatal, or 6- to 12-week-old fast and slow muscles, were analyzed for myosin and enolase isoforms as phenotypic markers. All clones derived from slow-oxidative muscles differentiated into myotubes with a preferentially slow contractile phenotype, whereas some clones derived from rapid-glycolytic or neonatal muscles expressed both fast and slow myosin isoforms. Thus, muscle origin appears to bias myosin isoform expression in myotubes. The neonatal clone (WTt) was cultivated in various medium and substrate conditions, allowing us to determine optimized conditions for their differentiation. Matrigel allowed expressions of adult myosin isoforms, and an isozymic switch from embryonic alpha- toward muscle-specific beta-enolase, never previously observed in vitro. These cells will be a useful model for in vitro studies of muscle fiber maturation and plasticity. Developmental Dynamics 237:1412-1423, 2008. (c) 2008 Wiley-Liss, Inc.

Domaines

Autre [q-bio.OT]

Dates et versions

hal-00281479 , version 1 (22-05-2008)

Identifiants

Citer

J. Peltzer, L. Colman, J. Cebrian, H. Musa, M. Peckham, et al.. Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro.. Developmental Dynamics, 2008, 237 (5), pp.1412-23. ⟨10.1002/dvdy.21543⟩. ⟨hal-00281479⟩
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