Pores Formation on Cell Membranes by Hederacolchiside A1 Leads to a Rapid Release of Proteins for Cytosolic Subproteome Analysis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Proteome Research Année : 2008

Pores Formation on Cell Membranes by Hederacolchiside A1 Leads to a Rapid Release of Proteins for Cytosolic Subproteome Analysis.

Gabriel Mazzucchelli
Nicolas Cellier
  • Fonction : Auteur
Vakhtang Mshviladzade
  • Fonction : Auteur
Riad Elias
  • Fonction : Auteur
  • PersonId : 172844
  • IdHAL : riad-elias
Yong-Ho Shim
  • Fonction : Auteur
David Touboul
Loïc Quinton
  • Fonction : Auteur
  • PersonId : 845257
Marie-Claire de Pauw-Gillet
  • Fonction : Auteur

Résumé

Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.
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Dates et versions

hal-00275499 , version 1 (24-04-2008)

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Citer

Gabriel Mazzucchelli, Nicolas Cellier, Vakhtang Mshviladzade, Riad Elias, Yong-Ho Shim, et al.. Pores Formation on Cell Membranes by Hederacolchiside A1 Leads to a Rapid Release of Proteins for Cytosolic Subproteome Analysis.. Journal of Proteome Research, 2008, 7 (4), pp.1683-1692. ⟨10.1021/pr7006973⟩. ⟨hal-00275499⟩
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