<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-00260689</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-02T20:48:51+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">17Beta-oestradiol up-regulates the expression of a functional UDP-glucose dehydrogenase in articular chondrocytes: comparison with effects of cytokines and growth factors.</title>
            <author role="aut">
              <persName>
                <forename type="first">L.</forename>
                <surname>Maneix</surname>
              </persName>
              <idno type="halauthorid">303805-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">G.</forename>
                <surname>Beauchef</surname>
              </persName>
              <idno type="halauthorid">303806-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">A.</forename>
                <surname>Servent</surname>
              </persName>
              <idno type="halauthorid">303807-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Y.</forename>
                <surname>Wegrowski</surname>
              </persName>
              <idno type="halauthorid">303808-0</idno>
              <affiliation ref="#struct-7569"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">F. X.</forename>
                <surname>Maquart</surname>
              </persName>
              <idno type="halauthorid">303809-0</idno>
              <affiliation ref="#struct-7569"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Noureddine</forename>
                <surname>Boujrad</surname>
              </persName>
              <idno type="halauthorid">124236-0</idno>
              <affiliation ref="#struct-804"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Gilles</forename>
                <surname>Flouriot</surname>
              </persName>
              <email type="md5">469d9af7bc269b2a0916b534e16a3f5b</email>
              <email type="domain">univ-rennes1.fr</email>
              <idno type="idhal" notation="string">gilles-flouriot</idno>
              <idno type="idhal" notation="numeric">180756</idno>
              <idno type="halauthorid" notation="string">7047-180756</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6250-4173</idno>
              <idno type="IDREF">https://www.idref.fr/121875792</idno>
              <affiliation ref="#struct-804"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">J. P.</forename>
                <surname>Pujol</surname>
              </persName>
              <idno type="halauthorid">303810-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">K.</forename>
                <surname>Boumediene</surname>
              </persName>
              <email type="md5">0bc8414561fc7f4377b794cb94f6fa06</email>
              <email type="domain">unicaen.fr</email>
              <idno type="idhal" notation="string">karim-boumediene</idno>
              <idno type="idhal" notation="numeric">1121336</idno>
              <idno type="halauthorid" notation="string">303811-1121336</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6517-8318</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">P.</forename>
                <surname>Galéra</surname>
              </persName>
              <idno type="halauthorid">303812-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">S.</forename>
                <surname>Moslemi</surname>
              </persName>
              <idno type="halauthorid">303813-0</idno>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Hervé</forename>
                <surname>De Villemeur</surname>
              </persName>
              <email type="md5">3b0c004a14295e4ca089a367e4e7ed40</email>
              <email type="domain">univ-rennes1.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2008-03-04 17:20:41</date>
              <date type="whenModified">2025-05-19 13:12:14</date>
              <date type="whenReleased">2008-03-04 17:20:41</date>
              <date type="whenProduced">2008-03</date>
              <ref type="externalLink" target="https://academic.oup.com/rheumatology/article-pdf/47/3/281/5028483/kem323.pdf"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="121134">
                <persName>
                  <forename>Hervé</forename>
                  <surname>De Villemeur</surname>
                </persName>
                <email type="md5">3b0c004a14295e4ca089a367e4e7ed40</email>
                <email type="domain">univ-rennes1.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-00260689</idno>
            <idno type="halUri">https://hal.science/hal-00260689</idno>
            <idno type="halBibtex">maneix:hal-00260689</idno>
            <idno type="halRefHtml">&lt;i&gt;Rheumatology&lt;/i&gt;, 2008, 47 (3), pp.281-8. &lt;a target="_blank" href="https://dx.doi.org/10.1093/rheumatology/kem323"&gt;&amp;#x27E8;10.1093/rheumatology/kem323&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Rheumatology, 2008, 47 (3), pp.281-8. &amp;#x27E8;10.1093/rheumatology/kem323&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UNIV-RENNES1">Université de Rennes 1</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="URCA">Publications de l'Université de Reims Champagne-Ardenne</idno>
            <idno type="stamp" n="IGDR">Institut Génétique et Développement de Rennes</idno>
            <idno type="stamp" n="UR1-UFR-SVE">UFR SVE Sciences de la vie et de l'environnement</idno>
            <idno type="stamp" n="UR1-HAL">Publications labos UR1 dans HAL-Rennes 1</idno>
            <idno type="stamp" n="UR1-SDV">UR1 - publications SDV</idno>
            <idno type="stamp" n="TEST-UNIV-RENNES">TEST Université de Rennes</idno>
            <idno type="stamp" n="TEST-UR-CSS">TEST Université de Rennes CSS</idno>
            <idno type="stamp" n="UNIV-RENNES">Université de Rennes</idno>
            <idno type="stamp" n="UR1-BIO-SA">Pôle UnivRennes - Biologie-Santé</idno>
            <idno type="stamp" n="TEST3-HALCNRS">TEST3-HALCNRS</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">17Beta-oestradiol up-regulates the expression of a functional UDP-glucose dehydrogenase in articular chondrocytes: comparison with effects of cytokines and growth factors.</title>
                <author role="aut">
                  <persName>
                    <forename type="first">L.</forename>
                    <surname>Maneix</surname>
                  </persName>
                  <idno type="halauthorid">303805-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">G.</forename>
                    <surname>Beauchef</surname>
                  </persName>
                  <idno type="halauthorid">303806-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">A.</forename>
                    <surname>Servent</surname>
                  </persName>
                  <idno type="halauthorid">303807-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Y.</forename>
                    <surname>Wegrowski</surname>
                  </persName>
                  <idno type="halauthorid">303808-0</idno>
                  <affiliation ref="#struct-7569"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">F. X.</forename>
                    <surname>Maquart</surname>
                  </persName>
                  <idno type="halauthorid">303809-0</idno>
                  <affiliation ref="#struct-7569"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Noureddine</forename>
                    <surname>Boujrad</surname>
                  </persName>
                  <idno type="halauthorid">124236-0</idno>
                  <affiliation ref="#struct-804"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Gilles</forename>
                    <surname>Flouriot</surname>
                  </persName>
                  <email type="md5">469d9af7bc269b2a0916b534e16a3f5b</email>
                  <email type="domain">univ-rennes1.fr</email>
                  <idno type="idhal" notation="string">gilles-flouriot</idno>
                  <idno type="idhal" notation="numeric">180756</idno>
                  <idno type="halauthorid" notation="string">7047-180756</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6250-4173</idno>
                  <idno type="IDREF">https://www.idref.fr/121875792</idno>
                  <affiliation ref="#struct-804"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">J. P.</forename>
                    <surname>Pujol</surname>
                  </persName>
                  <idno type="halauthorid">303810-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">K.</forename>
                    <surname>Boumediene</surname>
                  </persName>
                  <email type="md5">0bc8414561fc7f4377b794cb94f6fa06</email>
                  <email type="domain">unicaen.fr</email>
                  <idno type="idhal" notation="string">karim-boumediene</idno>
                  <idno type="idhal" notation="numeric">1121336</idno>
                  <idno type="halauthorid" notation="string">303811-1121336</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6517-8318</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">P.</forename>
                    <surname>Galéra</surname>
                  </persName>
                  <idno type="halauthorid">303812-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">S.</forename>
                    <surname>Moslemi</surname>
                  </persName>
                  <idno type="halauthorid">303813-0</idno>
                </author>
              </analytic>
              <monogr>
                <idno type="localRef">RA2007ACL08-MAN</idno>
                <idno type="halJournalId" status="VALID">11454</idno>
                <idno type="issn">1462-0324</idno>
                <idno type="eissn">1462-0332</idno>
                <title level="j">Rheumatology</title>
                <imprint>
                  <publisher>Oxford University Press (OUP)</publisher>
                  <biblScope unit="volume">47</biblScope>
                  <biblScope unit="issue">3</biblScope>
                  <biblScope unit="pp">281-8</biblScope>
                  <date type="datePub">2008-03</date>
                  <date type="dateEpub">2008-01-31</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1093/rheumatology/kem323</idno>
              <idno type="pubmed">18238796</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">UDP-glucose dehydrogenase</term>
                <term xml:lang="en">Glycosaminoglycan synthesis</term>
                <term xml:lang="en">17β-oestradiol</term>
                <term xml:lang="en">Oestrogen receptor</term>
                <term xml:lang="en">Cytokines</term>
                <term xml:lang="en">Cartilage</term>
                <term xml:lang="en">Chondrocytes</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.bbm.bm">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology</classCode>
              <classCode scheme="halDomain" n="sdv.bc">Life Sciences [q-bio]/Cellular Biology</classCode>
              <classCode scheme="halDomain" n="sdv.mhep.em">Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism</classCode>
              <classCode scheme="halDomain" n="sdv.mhep.rsoa">Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>OBJECTIVES: To investigate the mechanisms by which cytokines and 17beta-oestradiol (17beta-E2) modulate gene expression and activity of uridine diphosphoglucose dehydrogenase (UGDH), a key enzyme of GAG synthesis in articular chondrocytes. METHODS: Rabbit articular chondrocytes (RAC) from 3-week-old animals were incubated for 24 h with TGF-beta, insulin like growth factor-I (IGF-I), IL-1beta, IL-6 and 17beta-E2. GAG synthesis was measured by [35S]-sulphate labelling and the expression of the UGDH gene was estimated by both real-time polymerase chain reaction and western blotting, whereas the enzyme activity was assayed by a spectrophotometric procedure. In addition, the transcriptional activity of several UGDH gene promoter constructs was determined in RAC transiently transfected with wild-type or deleted human oestrogen receptor-alpha gene (hER alpha66 or hER alpha46, respectively). RESULTS: 17Beta-E2 and its receptor hER alpha66 enhanced GAG neosynthesis in rabbit articular chondrocytes, as did TGF-beta1 whereas IL-1beta decreased this synthesis. 17Beta-E2 was found to exert positive regulatory effects at mRNA, protein and UGDH activity levels. In addition, the receptor hER alpha66, but not hER alpha46, increased the transcriptional activity of the UGDH gene. In contrast, no clear correlation between transcription, translation and activity of the UGDH was found under the effects of the cytokines studied. However, TGF-beta enhanced the enzyme activity, whereas IL-1beta, IL-6 and IGF-I were without significant effect. CONCLUSIONS: 17Beta-E2 enhanced GAG synthesis in chondrocytes via up-regulation of the UGDH gene expression and enzyme activity. These data provide insights into the molecular mechanisms involved in the regulation of the UGDH gene and offer new approaches to investigate its potential alteration in joint diseases.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-7569" status="VALID">
          <idno type="IdRef">026403838</idno>
          <idno type="ISNI">0000000121767954</idno>
          <idno type="ROR">https://ror.org/03hypw319</idno>
          <orgName>Université de Reims Champagne-Ardenne</orgName>
          <orgName type="acronym">URCA</orgName>
          <desc>
            <address>
              <addrLine>2 avenue Robert Schuman, 51100 Reims.</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-reims.fr/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-804" status="OLD">
          <orgName>Interactions cellulaires et moléculaires</orgName>
          <orgName type="acronym">ICM</orgName>
          <date type="start">2000-01-01</date>
          <date type="end">2011-12-31</date>
          <desc>
            <address>
              <addrLine>Campus de Beaulieu, 263 avenue du Général Leclerc CS 74205 35042 RENNES CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://umr6026.univ-rennes1.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-105160" type="direct"/>
            <relation name="UMR6026" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-105160" status="VALID">
          <idno type="IdRef">26693823X</idno>
          <idno type="ROR">https://ror.org/015m7wh34</idno>
          <orgName>Université de Rennes</orgName>
          <orgName type="acronym">UR</orgName>
          <desc>
            <address>
              <addrLine>Campus de Beaulieu, 263 avenue Général Leclerc, CS 74205, 35042 RENNES CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.univ-rennes.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>