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Article Dans Une Revue Journal of Biological Inorganic Chemistry Année : 2007

A platinum-quinacridine hybrid as a G-quadruplex ligand.

Résumé

A novel platinum-quinacridine hybrid, comprising a monofunctional Pt moiety and a G-quadruplex ligand (mono-para-quinacridine or MPQ), has been synthesized and shown to interact with quadruplex DNA via a dual noncovalent/covalent binding mode. Denaturing gel electrophoresis was used to separate the various platination products of 22AG (an oligonucleotide that mimics the human telomeric repeat) by Pt-MPQ, and it was shown that two platinated adducts are highly stable quadruplex structures. Dimethylsulfate/piperidine treatment and 3'-exonuclease digestion of the isolated adducts allowed us to precisely determine the platination pattern of 22AG by Pt-MPQ, which displays three main sites G2, G10 and G22. Data presented herein support the hypothesis that Pt-MPQ traps preferentially the antiparallel structure of the 22AG quadruplex. Finally, the kinetics of Pt-MPQ platination using a construct containing both quadruplex DNA and a duplex DNA parts provide the first insights into the Pt-MPQ preference for quadruplex DNA over duplex DNA.

Domaines

Chimie organique

Dates et versions

hal-00259082 , version 1 (26-02-2008)

Identifiants

Citer

Hélène Bertrand, Sophie Bombard, David Monchaud, Marie-Paule Teulade-Fichou. A platinum-quinacridine hybrid as a G-quadruplex ligand.. Journal of Biological Inorganic Chemistry, 2007, 12 (7), pp.1003-14. ⟨10.1007/s00775-007-0273-3⟩. ⟨hal-00259082⟩
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