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Article Dans Une Revue Journal of Molecular Catalysis B: Enzymatic Année : 2008

Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4

Résumé

A semi-purified nitrile hydratase from Rhodococcus erythropolis A4 was applied to biotransformations of 3-oxonitriles 1a–4a, 3-hydroxy-2-methylenenitriles 5a–7a, 4-hydroxy-2-methylenenitriles 8a–9a, 3-hydroxynitriles 10a–12a and 3-acyloxynitrile 13a into amides 1b–13b. Cross-linked enzyme aggregates (CLEAs) with nitrile hydratase and amidase activities (88% and 77% of the initial activities, respectively) were prepared from cell-free extract of this microorganism and used for nitrile hydration in presence of ammonium sulfate, which selectively inhibited amidase activity. The genes nha1 and nha2 coding for α and β subunits of nitrile hydratase were cloned and sequenced.
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Dates et versions

hal-00204397 , version 1 (14-01-2008)

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  • HAL Id : hal-00204397 , version 1

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David Kubac, Ondrej Kaplan, Veronika Elisakova, Miroslav Patek, Vojtech Vejvoda, et al.. Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4. Journal of Molecular Catalysis B: Enzymatic, 2008, 50, pp.107-113. ⟨hal-00204397⟩
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