Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Vaccine Année : 2007

Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis.

David García-Yoldi
  • Fonction : Auteur
Clara M Marín
  • Fonction : Auteur
María J de Miguel
  • Fonction : Auteur
Pilar M Muñoz
  • Fonction : Auteur
Ignacio López-Goñi
  • Fonction : Auteur

Résumé

The assessment of the genetic stability is one of the essential elements to guarantee the biological quality of live anti-bacteria vaccines. Live attenuated Brucella melitensis Rev 1 is the most effective vaccine against brucellosis in small ruminants. Thirty-six B. melitensis Rev 1 vaccine strains isolated from human or animal sources from different geographic regions, from different commercial batches or laboratory collections were typed by the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) recently described for Brucella spp. Our results demonstrated that B. melitensis Rev 1 group as assayed by MLVA is genetically very homogeneous. We believe that MLVA methodology could be an essential assay to guarantee the quality and stability of live anti-bacterial vaccines being produced worldwide and can be included as in vitro control.

Dates et versions

hal-00195331 , version 1 (10-12-2007)

Identifiants

Citer

David García-Yoldi, Philippe Le Fleche, Clara M Marín, María J de Miguel, Pilar M Muñoz, et al.. Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis.. Vaccine, 2007, 25 (15), pp.2858-62. ⟨10.1016/j.vaccine.2006.09.063⟩. ⟨hal-00195331⟩
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