Communication Dans Un Congrès Année : 2007

Multimodal imaging to study the diffusion of a photosensitizer into tumoral tissue

Résumé

Photodynamic therapy (PDT) used for photodiagnostic and cancer treatment is based on interaction between a photosensitizer and light leading to tumoral tissue destruction. To perform the therapy, specific localisation and dose-ranging of photosensitive molecules should be optimised. The aim of this work was to follow the diffusion of a new photosensitizer according to time (30 min to 24 hours) into a tumor after an intratumoral injection. Multiphoton technology (infrared excitation) enables to image specimens in a non invasive mode with high resolution at deep penetration. The spectral imaging mode gives information about localization of fluorescent molecules and strongly depends on their concentration. Lifetime imaging mode gives access to their fluorescence decay which is independent on their concentration but sensible to their physicochemical environment. Second Harmonic Generation localizes the collagen content of specimens. Macrofluorescence imaging (visible excitation) enabled us to visualize the whole tumor and showed a progressive concentration of the photosensitizer in the tumoral tissue. Fluorescence detection methods (Spectral, FLIM, SHG) combined to a macroscopic observation represent major advanced in optical photodiagnostic. Based on these techniques, this work enabled us to determine the delay following injection for which the photosensitizer's concentration in tumoral tissue was optimised.

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Dates et versions

hal-00194215 , version 1 (06-12-2007)

Identifiants

  • HAL Id : hal-00194215 , version 1

Citer

Elisabethe Werkmeiser, Luc Marchal, Jean-François Stoltz, Yan Viry-Babel, Henri-Pierre Lassalle, et al.. Multimodal imaging to study the diffusion of a photosensitizer into tumoral tissue. 1er Forum du Cancéropôle du Grand-Est, Oct 2007, Vittel, France. ⟨hal-00194215⟩
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