Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies. - Archive ouverte HAL Access content directly
Journal Articles Acta crystallographica Section D : Structural biology [1993-...] Year : 2006

Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies.

Christophe Romier
Marouane Ben Jelloul
  • Function : Author
Shira Albeck
  • Function : Author
Gretel Buchwald
  • Function : Author
Patrick H N Celie
  • Function : Author
Evangelos Christodoulou
  • Function : Author
Valeria de Marco
  • Function : Author
Suzan van Gerwen
  • Function : Author
Puck Knipscheer
  • Function : Author
Joyce H Lebbink
  • Function : Author
Valerie Notenboom
  • Function : Author
Serge X Cohen
Tamar Unger
  • Function : Author
Joel L Sussman
  • Function : Author
Titia K Sixma
  • Function : Author
Anastassis Perrakis
  • Function : Author

Abstract

Structure determination and functional characterization of macromolecular complexes requires the purification of the different subunits in large quantities and their assembly into a functional entity. Although isolation and structure determination of endogenous complexes has been reported, much progress has to be made to make this technology easily accessible. Co-expression of subunits within hosts such as Escherichia coli and insect cells has become more and more amenable, even at the level of high-throughput projects. As part of SPINE (Structural Proteomics In Europe), several laboratories have investigated the use co-expression techniques for their projects, trying to extend from the common binary expression to the more complicated multi-expression systems. A new system for multi-expression in E. coli and a database system dedicated to handle co-expression data are described. Results are also reported from various case studies investigating different methods for performing co-expression in E. coli and insect cells.

Dates and versions

hal-00188127 , version 1 (15-11-2007)

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Christophe Romier, Marouane Ben Jelloul, Shira Albeck, Gretel Buchwald, Didier Busso, et al.. Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies.. Acta crystallographica Section D : Structural biology [1993-..], 2006, 62 (Pt 10), pp.1232-42. ⟨10.1107/S0907444906031003⟩. ⟨hal-00188127⟩
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